乙型肝炎血清标志物和HBV-DNA载量相关性分析

目的 探讨乙肝血清标志物与病毒DNA水平之间的相关性.方法 采用实时荧光定量PCR对874例HBV感染者血清中HBV-DNA含量进行检测,同时运用ELISA法检测HBV血清标志物,并统计分析患者乙型肝炎血清标志物、病毒DNA之间的相关性及分布特点.结果 在受检的874例标本中,男性533例,阳性率58.16％ （310/533）;女性341例,阳性率50.44％（172/341）,男性和女性阳性率比较差异有统计学意义（X2=5.01,P＜0.05）,男性和女性HBV-DNA水平差异无统计学意义（t =0.117,P=0.907）.乙肝总阳性率和HBV-DNA水平均随年龄的增长呈下降趋势,不同年龄组间比较差异有统计学意义.同一年龄段的大三阳与小三阳、两头阳和三抗阳之间比较差异有统计学意义;其中男性和女性HBV-DNA水平随年龄增长均呈下降趋势,差异有统计学意义.≤20岁以下人群HBV-DNA阳性率最高达82.86％.结论 HBV-DNA阳性率和HBV-DNA水平都随年龄增长呈下降趋势,其中≤20岁年龄段HBV-DNA阳性率最高达82.86％;男性HBV-DNA的阳性率高于女性.

Study on the correlation between serum markers of hepatitis B and HBV-DNA

Objective To investigate the correlation between HBV serum markers and HBV DNA levels. Methods HBV-DNA and serum markers in 874 cases of HBV infected persons were detected byreal-time fluorescence quantitative PCR and ELISA, respectively. SPSS 16. 0 was used to analyze the corre- lation and distribution characteristics. Results In 874 cases, 533 positive cases were male, the positive rate was 58.16% （310/533）. 341 positive cases were female, the positive rate was 50. 44% （172/341）. There was statistical difference （X2 = 5.01, P 〈 0.05 ） comparing male with female. But there was no statis- tical difference （t = 0. 117, P = 0. 907 ） in HBV-DNA level between male and female. The total positive rate and HBV-DNA level showed descending tendency with age, and there were statistical differences among different age groups. When comparing big 3 this world, small 3 this world, two head positive and three anti- body positive among the same age group, the statistical differences were found. The HBV-DNA level in both male and female showed descending tendency with age increasing, the statistical difference was found. 20 years old age group showed the highest positive rate （82. 86% ）. Conclusions The positive rate of HBV- DNA and HBV-DNA level deceased with the age increasing. ~〈 20 years old age group showed the highest HBV-DNA positive rate（ 82. 86% ）. HBV-DNA positive rate of the male was higher than the female.