HPLC测定三子降酶胶囊中三七皂苷R1和人参皂苷Rg1含量

目的：建立HPLC测定三子降酶胶囊中三七皂苷R。和人参皂苷Rg，的含量。方法：色谱柱ZorbaxSB—C18（250mm×4．6mm，5μm），流动相为乙腈一水梯度洗脱系统，流速为1．0mL·min-1，检测波长为203nm，柱温为25℃，进样量为10μL。结果：三七皂苷R1和人参皂苷Rg。分别在7．9～126．4μg·mL-1，25．6-409．6μg·mL-1线性关系良好。三七皂苷R1和人参皂苷Rg。平均回收率分别为98．43％，99．01％，RSD分别为1．7％，1．4％。结论：本方法简便、准确、重现性好，可用于三子降酶胶囊的质量控制。

Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Sanzi jiangmei Capsules by HPLC

Objective： To establish HPLC method for the determining of notoginsenoside R1 and ginsenoside Rgl in Sanzi jiangmei Capsules. Methods： The Zorbax SB-Cls column（250 mm x 4. 6 mm, 5μm） was used. The mobile phase was acetonitrile-water. The flow rate was 1.0 mL. min-1. The detection wavelength was 203 nm and column temperature was 25℃. Results： The linear range of notoginsenoside R1 and ginsenoside Rg1 were 7.9-126. 4μg-mL-1, 25.6-409.6μg＇mL-1 respectively. The average recovery rate of notoginsenoside R1 and ginsenoside Rg1 were 98. 43%, 99.01% （ RSD = 1.7%, 1.4% ） respectively. Conclusion： This method is simple, accurate and reproductive, which can be used for the quality control of Sanzi jiangmei Capsules.