Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests. |
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| Authors: | C H Carbonetto E L Malchiodi M Chiaramonte E Durante de Isola C A Fossati and R A Margni |
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| Institution: | Institute of Humoral Immunity Studies (IDEHU) CONICET/UBA, Department of Immunology, Faculty of Pharmacology and Biochemistry, Argentina. |
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| Abstract: | By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes. |
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| Keywords: | Trypanosoma cruzi antigen Chagas' disease serological tests monoclonal antibodies |
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