胶束电动毛细管色谱法测定西洋参中人参皂苷Rb1,Re的含量

目的 :建立西洋参中人参皂苷Re ,Rb₁的胶束电动毛细管色谱测定方法 ,考察影响分离的各因素 ,确定最佳色谱条件。方法 :测定缓冲液为含 20mmol·L^-1硼酸、2 0mmol·L^-1硼砂、6 0mmol·L^-1胆酸钠和 20%乙腈的混合溶液。电压 20kV ,柱温 25℃ ,检测波长 203nm ,压力进样 5s。结果 :线性范围Re为 0.38～1.65mmol·L^-1,Rb₁为 0.42～ 1.76mmol·L^-1。加样回收率Re为 97.2% ,RSD =1.6% ;Rb1为 97.7% ,RSD =1.9% (n=5 )。结论 :该方法具有样品前处理简单 ,重现性好 ,所得色谱图信息丰富等特点。

Determination of Ginsenosides Re,Rb1 in Panax quinquefolius by Micellar Electrokinetic Chromatography

OBJECTIVE: A Micellar electrokinetic chromatography (MEKC) technique for the determination of ginsenosides Re, Rb1 in Panax quinquefolius was developed and validated. METHOD: The MEKC was performed in a mixed buffer solution containing 20 mmol x L(-1) boric acid, 20 mmol x L(-1) sodium tetraborate, 60 mmol x L(-1) sodium cholate (CA) and 20% acetonitrile under the applied voltage of 20 kV at 25 degrees C. The detection wavelenth was 203 nm, the sampling time is 5 sec (hydrostatic injection). RESULT AND CONCLUSION: The liner range was 0.38 - 1.65 mg x ml(-1) for Re and 0.42 - 1.76 g x L(-1) for Rb1. The average recovery for Re was 97.2%, RSD = 1.6% and that for Rb1 was 97.7%, RSD = 1.9% (n = 5). The preparation of sample is easy and the chromatogram has much information.