BDNF、TrkB和E-cadherin在唾液腺腺样囊性癌中的表达及意义

目的 研究BDNF、TrkB和E-cadherin在唾液腺腺样囊性癌（salivary adenoid cystic carcinoma,SACC）中的表达及其与临床病理特点的关系。方法：采用免疫组织化学染色检测76例唾液腺腺样囊性癌和20例正常唾液腺组织中BDNF、TrkB和E-cadherin的表达情况,并分析其与临床病理特征之间的关系。采用SPSS17.0软件包对实验结果进行χ²检验、Wilcoxon秩和检验、Spearman相关系数检验。结果：与正常唾液腺组织相比,BDNF、TrkB在SACC组织中呈现高表达,阳性表达率分别为89.5%、92.1%;E-cadherin呈现中低表达,阳性表达率为46.1%。BDNF的表达与肿瘤临床分期、肿瘤转移显著相关（P<0.05）。TrkB的表达与肿瘤临床分期、神经侵袭、肿瘤转移显著相关（P<0.05）。 E-cadherin的表达与肿瘤病理分型、临床分期、神经侵袭、转移呈显著负相关（P<0.05）。在SACC中,BDNF表达与 E-cadherin表达无显著相关性（P>0.05）,而TrkB表达与E-cadherin表达呈显著负相关（P<0.05）。结论：BDNF、TrkB和E-cadherin的表达与SACC的临床病理特征密切相关,检测3者的表达对SACC的诊断和临床病理研究具有重要的参考价值。

Expression and significance of BDNF and TrkB and E-cadherin in salivary adenoid cystic carcinoma

PURPOSE： To investigate the expression of brain-derived neurotrophic factor（BDNF）, tropomysin-related kinase B（TrkB） and E-cadherin in salivary adenoid cystic carcinoma（SACC） and its correlation with clinicopathological characteristics. METHODS： BDNF, Trkb and E-cadherin expression in 76 primary SACC cases and 20 normal salivary gland tissues were analyzed by immunohistochemical staining. The results and clinicopathological data were statistically analyzed by SPSS17.0 software package with χ2test, Wilcoxon rank sum test, and Spearman’s rank correlation coefficient test. RESULTS： High expression of BDNF（89.5%） and TrkB（92.1%） and low expression of E-cadherin（46.1%） were found in SACC cases. The expression of BDNF was significantly associated with the clinical stages and distance metastasis（P 〈0.05）. The expression of TrkB was significantly associated with the clinical stage, perineural invasion and distance metastasis（P〈0.05）. The expression of E-cadherin was significantly associated with the clinical stages, histopathological types, perineural invasion and distance metastasis（P〈0.05）. There was no significant correlation between the expression of BDNF and E-cadherin（P〉0.05）. However, the expression of TrkB was negatively correlated with the expression of Ecadherin（P 〈0.05）. CONCLUSION： The expression of BDNF, TrkB and E-cadherin was closely correlated to the clinicopathological characteristics of SACC. The detection of BDNF, TrkB and E-cadherin expression may be of great significance for clinicopathological study and diagnosis of SACC.