糖脂平对胰岛素抵抗大鼠骨骼肌氧化应激的影响

目的建立喂养型胰岛素抵抗（insulinresistance，IR）大鼠模型，观察糖脂平对喂养型IR大鼠氧化应激的影响。方法选用体重在180～200g的8周龄雄性SD大鼠60只，适应性喂养一周后，随机分为空白对照组15只和造模组45只。空白对照组采用普通饲料喂养，造模组采用高脂饲料喂养。8周造模成功后，将造模组随机分为：模型组、糖脂平组、罗格列酮组各15只。糖脂平组给予糖脂平按生药20g/kg·d灌胃；罗格列酮组给予罗格列酮0．8mg／kg·d灌胃；模型组和空白对照组灌服等量的生理盐水；末次给药后禁食12小时，用正常血糖胰岛素钳夹技术评价胰岛素敏感性，检测各组大鼠的体重、血糖、血清胰岛素水平，并留取骨骼肌，检测组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）、丙二醛（MDA）、过氧化氢酶（CAT）等指标。结果糖脂平能增加胰岛素敏感性，改善胰岛素抵抗，糖脂平组的M值显著高于模型组，体重、血糖、胰岛素均低于模型组，差异有统计学意义（P〈0．05），与罗格列酮组比较，体重一项有统计学意义（P〈0．05），血糖与胰岛素无统计学意义（P〉0．05）；糖脂平组SOD、GSH—Px、CAT水平显著高于模型组，MDA显著低于模型组，差异有统计学意义（P〈0．05），与罗格列酮组比较，差异无统计学意义（P〉0．05）。结论糖脂平可改善胰岛素抵抗，也可改善氧化应激，反应了糖脂平可以通过改善胰岛素抵抗的上游通路——氧化应激来提高机体代谢活力，改善胰岛素抵抗，其作用机制可能是通过改善骨骼肌中线粒体的活性氧。

Effects of Tangzhiping on oxidative stress in rat insulin resistance model

Objective To investigate the effects of Tangzhiping on oxidative stress in rats with in- sulin resistance. Methods 60 male SD rats （weighs 180 -200 g, ages 8 w） were selected and fed a week for adaptation. Then, the rats were randomly divided into blank control group （ n = 15 ） and model group （ n = 45 ）. Blank control group were fed with common food, while model group were fed with high fat food, last for 8 weeks. After 8 weeks the mould was successfully made, these modules were randomly divided in- to ： the model group, Tangzhiping group, rosiglitazone group （ n = 15 in each group）. Tangzhiping group were given Tangzhiping formula particles according to the statement 20 g/kg · d to fill in the stomach; Ros- iglitazone group were given 0. 8 mg/kg · d rosiglitazone gavage; Model group and blank control group were given the same amount of saline solution, again for 8 weeks ; 12hs after the last administration of fasting, levels of insulin sensitivity were evaluated by the ＂euglycemic hyperinsulin clamp technique＂, and levels of body weight, fast plasma glucose, serum insulin , were observed, Meanwhile superoxide dismutase （ SOD）, glutathione peroxidase （GSH-Px） malonaldehyde （ MDA ）, and catalase （CAT） of skeletal muscle were measured. Results The M level was significantly higher while mean body weight, blood glucose, and fasting insulin of Tangzhiping group were lower than that of model group,. The differences were statisti- cally significant （ P 〈 0. 05 ）, with rosiglitazone group, which body weight was statistically significant （ P 〈 0.05） , blood sugar and insulin had no statistical significance （P 〉 0. 05） ; The mean SOD, GSH and CAT level of Tangzhiping group is significantly higher than model group, MDA level was lower, the differences were statistically significant （ P 〈 0. 05 ）, with rosiglitazone group statistically significant （ P 〈 0. 05 ）. Con- clusion Tangzhiping can improve insulin resistance, and also improve oxidative stress, which reflects that Tangzhiping can enhance body＇s metabolic activity through improving the upstream pathway of IR--oxidative stress, in the end improve IR. Its mechanism may be through improving skeletal muscle mitochondrial ROS.