RNA干扰TEAD基因对舌癌细胞系Tca8113增殖和侵袭的影响

目的：应用RNA干扰技术抑制人舌癌细胞株Tca8113中内源TEAD基因的水平，观察TEAD基因对舌癌细胞生物学行为的影响。方法：构建针对TEAD基因特异性siRNA真核表达载体，将其转染至Tea8113，采用RT—PCR法检测转染后的Tca8113细胞中TEAD基因的表达。采用CCK8技术检测细胞的增殖情况，采用Transwell法检测细胞的迁移情况。实验数据采用SPSSl3．0软件包进行单因素方差分析。结果：siRNA干扰Tea8113细胞后，TEAD基因的表达水平显著下降（P〈0．05），细胞生长缓慢，体外侵袭能力下降。结论：通过RNA干扰技术阻断TEAD的表达，可抑制，rca8113细胞的生长、增殖、迁移，提示TEAD基因在舌癌的发生、发展过程中起着重要作用。

Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line

PURPOSE： To investigate the effect of TEAD gene on biological behavior of tongue squamous cell carcinoma by endogenous TEAD gene knockdown using RNA interference technique in the tongue carcinoma cell line Tca8113. METHODS： Specific iRNA of TEAD gene was established and transfected into the cell line Tca8113. The TEAD gene expression in Tca8113 was detected after transfection using RT-PCR. Cell proliferation and migration were observed by CCK8 and Transwell methods, respectively. The experimental data was statistically analyzed by One-Way ANOVA with SPSS13.0 software package. RESULTS： After siRNA interference, Tca8113 cell line showed significantly decreased expression of TEAD gene （P〈0.05）, lower rate of proliferation （P〈0.05）, and weakened invasion （P〈0.05） in vitro. CONCLUSION： The inhibition of TEAD gene expression by RNAi can suppress the growth, proliferation and migration of Tca8113 cell line, indicating a pivot role of TEAD gene in the pathogenesis of tongue squamous cell carcinoma. Supported by Science and Technology Development Fund of Nanjing Medical University（2013NJNU158）.