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| 正加速度暴露对实验性胃溃疡大鼠胃黏膜CGRP-mRNA表达和胃黏膜血流量的影响 | |
| 引用本文： | 赵锟,陈英,李静,范勤,唐合兰,杨春敏,王建昌. 正加速度暴露对实验性胃溃疡大鼠胃黏膜CGRP-mRNA表达和胃黏膜血流量的影响[J]. 军医进修学院学报, 2015, 36(1): 75-78 |
| 作者姓名： | 赵锟 陈英 李静 范勤 唐合兰 杨春敏 王建昌 |
| 作者单位： | 1. 空军总医院干部病房消化科,北京,100142 2. 空军总医院麻醉科,北京,100142 3. 空军总医院医院办公室,北京,100142 |
| 基金项目： | 全军十二五后勤科研计划基金资助项目(AKJ11J004)Supported by the 12th Five Years Major Scientific Research Program of General Logistics Department of PLA |
| 摘 要： | 目的研究正加速度(positive acceleration,+Gz)暴露对实验性胃溃疡大鼠胃黏膜降钙素基因相关肽mRNA(calcitonin gene-related peptide-mRNA,CGRP-mRNA)的表达及胃黏膜血流(gastric mucosal blood flow,GMBF)的影响。方法选择雄性SD大鼠40只随机分成4组,每组10只,A组为正常对照组,不做处理;对B、C、D组大鼠采用乙酸烧灼法建立胃溃疡模型,造模3 d后加速度处理大鼠,B组地面捆绑5 min(相当于+1 Gz恒速暴露),C组+5 Gz旋转5 min,D组+10 Gz旋转5 min,隔日1次,共4次。1周后麻醉大鼠并固定,用MoorVMS-LDF2型激光多谱勒血流仪测定GMBF;采集胃黏膜组织,用荧光定量PCR的方法定量测定胃黏膜CGRP-mRNA含量(2~-△△ct)。结果胃黏膜CGRP-mRNA的含量,B组(3.090±0.154)较A组(0.966±0.089)升高(P<0.05);+Gz暴露后,B组>C组(2.262±0.116)>D组(1.096±0.077),差异均有统计学意义(P<0.05)。GMBF量,A组(432.919±11.598)pU>B组(367.602±10.133)pu>C组(303.925±16.417)pu>D组(246.750±22.815)pu,差异均有统计学意义(P<0.05)。胃溃疡大鼠(B组、C组、D组大鼠)+Gz暴露后,CGRP-mRNA表达和GMBF量存在正相关(r=0.807,P<0.05)。结论 +Gz暴露导致实验性大鼠胃溃疡愈延迟合,其可能与+Gz条件下胃黏膜血流量减少及胃黏膜CGRP-mRNA的表达改变有关。 |
| 关 键 词： | 正加速度 胃溃疡 胃黏膜血流 降钙素基因相关肽mRNA |
Effects of positive acceleration on the expression of CGRP-mRNA in gastric mucosa and gastric mucosal blood flow of rats with experimental gastric ulcer |
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| ZHAO Kun,CHEN Ying,LI Jing,FAN Qin,TANG Helan,YANG Chunmin,WANG Jianchang. Effects of positive acceleration on the expression of CGRP-mRNA in gastric mucosa and gastric mucosal blood flow of rats with experimental gastric ulcer[J]. Academic Journal of Pla Postgraduate Medical School, 2015, 36(1): 75-78 | |
| Authors: | ZHAO Kun CHEN Ying LI Jing FAN Qin TANG Helan YANG Chunmin WANG Jianchang |
| Affiliation: | ZHAO Kun;CHEN Ying;LI Jing;FAN Qin;TANG Helan;YANG Chunmin;WANG Jianchang;Cadres Ward;Department of Gastroenterology General Hospital of Air Force of Chinese PLA;Department of Anesthesiology General Hospital of Air Force of Chinese PLA;The Hospital Office General Hospital of Air Force of Chinese PLA; |
| Abstract: | |
| Keywords: | positive acceleration stomach ulcer gastric mucosal blood flow calcitonin gene-related peptide-mRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |