Time-dependent antimicrobial effect of photodynamic therapy with TONS 504 on <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> |
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Authors: | Kentaro Sueoka Taiichiro Chikama Miftahul Akhyar Latief Ji-Ae Ko Yoshiaki Kiuchi Takemasa Sakaguchi Akira Obana |
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Institution: | 1.Department of Ophthalmology and Visual Science, Graduate School of Biomedical Sciences,Hiroshima University,Hiroshima,Japan;2.Department of Ophthalmology,Hasanuddin University,Makassar City,Indonesia;3.Department of Virology, Graduate School of Biomedical Sciences,Hiroshima University,Hiroshima,Japan;4.Department of Ophthalmology,Seirei Hamamatsu General Hospital,Shizuoka,Japan |
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Abstract: | Pseudomonas aeruginosa (P. aeruginosa) is a major cause of infectious keratitis, which itself is a major cause of blindness worldwide. We have now evaluated the time-dependent effectiveness of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS 504 and a light-emitting diode (LED) on P. aeruginosa in vitro. PACT with TONS 504 (10 mg/L) and irradiation (30 J/m2) by an LED device that delivers light centered on a wavelength of 660 nm was applied to 1?×?106 colony-forming units of P. aeruginosa in liquid medium. The bacteria were then cultured at 37 °C for various times before assay of viability by determination of colony formation on agar plates. The effect of a second irradiation at 3 h after the initial LED exposure was also examined. Bacterial growth was markedly inhibited between 3 and 9 h after PACT with TONS 504, with the maximal effect being apparent at 3 h. Furthermore, a second exposure to LED irradiation at 3 h after the first treatment enhanced the inhibitory effect on bacterial growth. PACT with TONS 504 thus inhibited the growth of P. aeruginosa in a time-dependent manner, and an additional irradiation exposure applied 3 h after the first LED treatment greatly increased the effectiveness of PACT. This antibacterial system thus warrants further evaluation with regard to its potential effectiveness for the treatment of infectious keratitis. |
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