标本采集管与保存方式对超氧化物歧化酶测定结果的影响

目的：探讨标本采集管与保存方式对超氧化物歧化酶（SOD）测定结果的影响。方法：随机选取湖北科技学院附二医院80例体检者,前40例体检者同时分别用分离胶促凝真空管（标记为A组）和无抗凝真空管（标记为B组）采集血液标本,离心分离血清,两组都放室温,A组分别于0、4、6、8、10 h测定SOD活性,B组于0 h检测SOD活性;后40例体检者用分离胶促凝真空管（标记为C组）采集血液标本,离心分离血清,置于2-8℃冰箱下保存,分别于0、10、14、21 h测定SOD活性。采用配对t检验分析各组结果差异性。结果：分离胶促凝真空管和无抗凝真空管血清的SOD比较（0 h）,差异无统计学意义（P〉0.05）;室温下保存的分离胶促凝真空管血清（A组）的SOD于4、6、8、10 h检测,与0 h比较差异具有统计学意义（P〈0.01）,与0 h比较相对偏倚分别为3.719%、5.341%、6.807%、8.306%;2-8℃冰箱保存的分离胶促凝真空管血清（C组）的SOD于10、14、21 h检测,与0 h比较差异具有统计学意义（P〈0.01）,与0 h比较相对偏倚分别为3.213%、4.552%、5.816%。结论：可采用分离胶促凝真空管采集标本;室温下放置10 h及2-8℃冰箱放置21 h对结果的影响均在临床可接受范围内,与产品说明书上要求的时间相比,室温与冰箱中放置时间都能延长1倍以上。

Influence of specimen collection tube and preservative way on the determination results of superoxide dismutase

Objective：To explore the influence of specimen collection tube and preservative way on the determination results of superoxide dismutase（ SOD）. Methods： 80 subjects were randomly selected from the Second Affiliated Hospital,Hubei University of Science and Technology. Firstly,40 subjects blood specimens were simultaneously put in separation gel coagulant vacuum tubes（marked as A group）and in no anticoagulation vacuum tubes（marked as B group）. Secondly,40 subjects blood specimens were put in separation gel coagulant vacuum tubes（marked as C group）. After centrifugal separation,A group serum was preserved at room temperature,then the SOD was detected respectively at 0 h,4 h,6 h,8 h and 10 h. B group serum was preserved at room temperature,then the SOD was detected at 0 h. C group serum was preserved at 2-8 ℃ refrigerator,then the SOD was detected respectively at 0 h,10 h,14 h and 21 h. Results： The differences of the determination of SOD between separation gel and no anticoagulation vacuum tubes had no statistically significant（ P 0. 05）. But the determination results of SOD,which were detected in separation gel coagulant vacuum tubes after 4 h,6 h,8 h,10 h at room temperature,were statistically significantly different from the ones detected at 0 h（ P 0. 01）,and the relative bias of 4 h,6 h,8 h,10 h were 3. 719%,5. 341%,6. 807%,8. 306% respectively in comparison with 0 h. So were specimens in no anticoagulation vacuum tubes and preserved at 2-8 ℃ refrigerator,the relative bias of 10 h,14 h,21 h were3. 213%,4. 552%,5. 816% respectively in comparison with 0 h. Conclusion： Firstly,it is reasonable to use separation gel coagulant vacuum tubes for the specimen collection. Secondly,the determination results of specimens in separation gel coagulant vacuum tubes,both the ones after 10 h at room temperature and the ones after 21 h at2-8 ℃ refrigerator are in the clinical acceptable range. The preservative time can be prolonged by more than 1times compared with the required time in the product specification.