加减防己黄芪汤对糖尿病皮肤溃疡大鼠血清NO、ET-1水平的影响

目的]观察加减防己黄芪汤对糖尿病皮肤溃疡大鼠血管内皮功能的影响。方法]选用SD雄性大鼠90只,随机选取6只为正常对照组,其余大鼠以尾静脉注射四氧嘧啶40mg/kg,选取符合糖尿病诊断标准的大鼠用于建立糖尿病皮肤溃疡模型造模,并随机分成6组,模型对照组、加减防己黄芪汤干预的高、中、低剂量组,中药对照组、西药对照组。实验结束后断头取血检测NO、ET-1含量以加减防己黄芪汤对观察对血管内皮功能的影响。结果]与模型组比较,各治疗组大鼠血清NO含量均有不同程度升高,而血浆ET-1含量却有不同程度的降低,其中以加减防己黄芪汤高剂量组变化最明显(P0.001),中剂量明显(P0.01),低剂量、中药对照组次之(P0.05)。加减防己黄芪汤各剂量组的调节作用与剂量呈现相关性。结论]加减防己黄芪汤可通过升高血清NO含量、降低血浆ET-1水平来改善血管内皮功能。

Effect of Revised Astragalus Tetrandrae Decoction on Rats Serum NO and ET-1 with Diabetic Derma Ulcer

Objective]To explore the cure effect of Astragalus Tetrandrae Decoction on vessel endodermis function of rats with diabetic derma ulcer.Method]Select 90 SD male rats,take 6 as normal control group,others were injected by tail vein with Alloxan;choose rats in accordance with diabetic diagnosis to set up model of diabetic derma ulcer,and randomly divide them into 6 groups：model control（1）,high,middle and low-dosage groups（2,3,4）of Astragalus Tetrandrae Decoction,TCM control（5）and WM control（6）;after test,make decapitated and take blood for NO and ET-1 to observe effect of the decoction on vessel endodermis.Result]Compared to model group,all NO in treated groups was raised in different extent,While plasma ET-1 content reduced in different degree;among which,group 2 was the most obvious,then groups 3 and 5;the regulation was related with dosage.Conclusion]The decoction can improve vessel endodermis function by raising NO and reducing ET-1 level.