猪脾脏中的应激免疫抑制蛋白类似物(英文)

目的:研究猪脾脏中的一种具有免疫抑制活性的蛋白质,纯化该蛋白并研究其与临床疾病的关系。方法:1)稀酸提取;2)超滤透析分离分子量大于M_r30 000的分子;3)FPLC免疫亲和层析纯化;4)T淋巴细胞转化及ELISA检测其功能活性;5)SDS-PAGE检测其分子量及纯度。结果:该蛋白能抑制由Con A诱导的淋巴细胞增殖。其分子量在190 000 左右。该蛋白对某些来源于免疫细胞系的细胞,具有明显的选择性抑制作用。结论:成功部分纯化这种蛋白,并证明其与应激免疫抑制蛋白有相似的理化、免疫原性和功能活性。这一研究为进一步大量纯化该蛋白提供了实用和稳定的生物学来源,并为研究它与肿瘤、自身免疫性等临床疾病关系开辟了一条新的研究途径。

A protein in pig spleen similar to immune suppressive protein of stress

AIM: To purify a protein in pig spleens, which was similar to immune suppressive protein of stress (ISPS), and characterize its properties and functions. METHODS: 1) Pig spleen was extracted in dilute hydrochloric acid. 2) The extract was ultra-filtrated for having high molecular weight proteins (M_r>30000). 3) The filtrates were purified with FPLC affinity chromatography. 4) The elute from FPLC was used for T-lymphocyte proliferation and ELISA test. 5) Lastly, SDS-PAGE was used to determine the molecular weight and purity of the final product. RESULTS: A protein purified from pig spleen (the pig ISPS homologue) inhibited concanavalin A (Con A)-induced mouse lymphocyte proliferation. The molecular weight of this protein was about M_r 190000. It has a stronger selectivity against T-lymphocyte line such as Jurkat cell line and mastocyte line (F815) and has a weaker inhibitory activity on macrophage line(U937). CONCLUSION: A protein similar to rat/mouse ISPS was found in pig spleen. This may provide an opportunity to study its roles in tumors and autoimmune diseases.