灯盏花乙素对脑缺血再灌注损伤大鼠肝功能的影响

目的:观察灯盏花乙素对脑缺血再灌损伤大鼠肝功能的影响。方法:大鼠灌胃给药7日后,阻塞大脑中动脉诱导脑缺血再灌损伤,检测大鼠血清和/或肝组织中的一氧化氮、黄嘌呤氧化酶、丙氨酸转氨酶、天冬氨酸转氨酶、丙二醛、抗氧化酶、及细胞色素P-450活性(水平)。结果:灯盏花乙素明显降低(P<0.01)血清中升高的黄嘌呤氧化酶、丙氨酸转氨酶、天冬氨酸转氨酶及肝细织中的丙二醛(P<0.05),升高血清(P<0.01)和肝组织(P<0.05)中降低的一氧化氮并可诱导超氧化物歧化酶(P<0.05)和谷胱甘肽过氧化物酶(P<0.01),除明显降低(P<0.01) CYP3A的活性外,大鼠脑缺血再灌损伤对肝CYP1A1、CYP1A2、CYP2E1的活性没有影响,灯盏花乙素不影响脑缺血再灌损伤大鼠肝CYP的活性。结论:大鼠脑缺血再灌可诱发肝细胞损伤,灯盏花乙素具有保护作用,其作用机制与抗氧化有关。

Effects of scutellarin on liver function after brain ischemia/reperfusion in rats

AIM: To investigate the effects of scutellarin(Scu) on liver function after brain ischemia/reperfusion in Wistar rats. METHODS: Rats were pretreated with Scu for 7 d and then subjected to a brain ischemia/reperfusion injury induced by a middle cerebral artery occlusion. The levels of nitric oxide(NO), xanthine oxidase(XOD), alanine transaminase(ALT), and aspartate aminotransferase(AST) in serum or liver tissues and the activities of antioxidant enzymes and cytochrome P450-dependent monooxygenases(CYPs) in liver tissues after brain ischemia/reperfusion were determined. RESULTS: In vehicle-treated rats, XOD, ALT, and AST activities(P<0.01) in serum and the MDA level (P<0.05) in liver tissues were all elevated but were significantly reduced(P<0.05) by Scu pretreatment. The NO levels in serum and liver tissues were decreased(P<0.01) dramatically in vehicle-treated rats and returned to the levels in the sham-operated animals when pretreated with Scu. SOD (P<0.05) and GSH-PX(P<0.01) activities in cytosol fraction were increased significantly by Scu pretreatment. Furthermore, a loss of CYP3A activity (P<0.01), but no changes of CYP1A1, CYP1A2, and CYP2E1 activities in liver were observed after brain ischemia/reperfusion in rats. Scu had no effect on them. CONCLUSION: These results demonstrated that pretreatment with Scu could attenuate hepatocellular damage elicited by brain ischemia/reperfusion in rats and this protection is in major part by its antioxidant activity.