扇贝多肽对紫外线B所致人真皮成纤维细胞凋亡和DNA损伤的抑制作用

目的:研究扇贝多肽(PCF)对中波紫外线(UVB)所致体外培养人真皮成纤维细胞的凋亡和DNA损伤的影响。方法:用噻唑兰(MTT)法检测成纤维细胞的存活率;流式细胞术测定细胞的凋亡率和胞浆内游离Ca~(2+)]_i;彗星电泳法检测单细胞DNA受损的程度。结果:PCF(0.25％-1％)能显著提高UVB(1.176×10~(-4) J·cm~(-2))辐射后人真皮成纤维细胞的增殖活性,降低UVB所致细胞的凋亡率和死亡率,减轻受损的程度,同时可降低胞浆内游离Ca~(2+)]_i(和UVB模型组相比,P<0.01).结论:PCF对抗UVB所致的光老化是基于其减轻UVB对DNA的损伤和降低UVB所致细胞的凋亡,因此,PCF能够在起始阶段阻止紫外线所致光老化的发展。

Inhibitory effect of polypeptides from Chlamys farreri on UVB-induced apoptosis and DNA damage in normal human dermal fibroblasts in vitro

AIM: To investigate the effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B (UVB)-induced apoptosis and DNA damage in cultured normal human dermal fibroblasts. METHODS: MTT assay was used to measure the viability of cells. Measurements of apoptosis and cytosolic free Ca2+]i were performed with flow cytometry. The comet assay was employed to detect DNA damage in individual cell. RESULTS: PCF (0.25 %-1%) greatly enhanced the proliferative capacity of cultured fibroblasts irradiated by UVB (1.176 x 10(-4) J/cm2) and markedly reduced apoptosis and the level of DNA damage in a concentration-dependent manner. Meanwhile, PCF could decrease the cytosolic free Ca2+]i (P<0.01, compared with UVB model). CONCLUSION: The inhibitory effect of PCF on UVB-induced photoaging is due to enhanced abating of UVB-injured DNA and UVB-induced apoptosis. Therefore, PCF can resist UV-induced aging development at the initiation stage.