不同表型双价痢疾菌苗滴鼻免疫小鼠后黏膜和系统记忆反应观测

目的：观察5株2类不同表型的双价痢疾菌苗滴鼻免疫后所引起的黏膜和系统的记忆反应，为痢疾菌苗的构建提供理论依据。方法：以小鼠滴鼻免疫为模型，3次免疫（间隔2周）后，20周再次免疫，分别于3次免疫及20周加强免疫后第7天，收取鼻咽、肺、小肠及阴道冲洗液和血清，应用ELISA方法检测福氏、宋内氏特异性抗体。结果：2类双价菌苗株滴鼻免疫3次后，多个黏膜部位及血清均产生了抗原特异性sIgA、IgG，较PBS对照组有明显升高；20周后再次加强免疫，仍可诱导黏膜及系统的免疫反应，但较3次免疫后水平明显降低。结论：2株双价痢疾菌苗滴鼻免疫小鼠后可诱导多个黏膜及系统的特异性抗体产生，并可产生一定时间的记忆反应，侵袭蛋白与免疫记忆有关。

Detection of memory responses of specific antibodies in local mucosa and serum after intranasal immunization with two bi-valent Shigella vaccines in mice

Objective FSM 2117,FS 5416,FS 5414,FS 5411 and FS 5402 are two types of bivalent hybrid strains of S. flexneri and S. sonnei, constructed by our laboratory, The FS 5416,FS 5414,FS 5411 and FS 5402 express four invasive plasmid antigens and can be invasive to Hela cells(inv+), but the FSM 2117 does not(inv-). To observe the differences of the two types of bivalent shigella vaccines in immune responses and memory responses, we detected the antibodies of shi gella flexneri and sonnei LPS after three times immunization and boosting immunization after 20 weeks.Methods Using the ELISA assay, the changes of specific antibodies of mice (the levels of SIgA and IgG) were detected after immunization with five different vaccines and boosting immunization after an interval of 20 weeks. Results We observed significant rises in different mucosal sites wash and serum on the two times immunization.Significant differences were observed between two immunized groups and the control. Both of the two types of bivalent shigella vaccines showed memory response, but the levels of antibodies were lower than that of the third immunization. Conclusion Two types of shigella vaccines can induce antigen specific antibodies not only in several mucosal sites but also in serum after three times immunization. They can arose the memory immune responses not only in local nasal site but also in the other mucosal sites and serum. The expression of invasive plasmid antigens can improve the level of antibodies immune responses and the memory responses.