银杏提取物治疗血管成形术后再狭窄的机制研究

目的:观察银杏提取物(GBE)对血管成形术后血管内皮损伤再狭窄的作用,并探讨其可能机制。方法:40只兔建立髂动脉粥样硬化性狭窄模型后,随机分为4组灌胃治疗(n均=10):(1)模型对照组,于经皮经腔血管成形术(PTA)后给予生理盐水;(2)低剂量组,给予PTA兔GBE0.16g/kg/日;(3)中剂量组,给予PTA兔GBE0.32g/kg/日;(4)高剂量组,给予PTA兔GBE0.48g/kg/日;另10只实验兔普通食料喂养作为空白对照组。4周后检测各组动物血清低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、总胆固醇(TC)、血小板活化因子(PAF)、超氧化物歧化酶(SOD)、脂质过氧化物(LPO)、丙二醛(MDA)水平以及组织c-sis基因和c-myc蛋白表达。结果:与空白对照组比较,模型对照组的血清学指标有显著差异(P<0.05)。与模型对照组相比,各实验组血清LDL-C、TC、LPO、MDA、PAF水平,组织c-sis基因和c-myc蛋白表达均明显下降(P<0.05),而HDL-C、SOD水平明显升高(P<0.05)。中剂量组和高剂量组之间所有血清学指标以及c-myc蛋白表达的差异无显著性(P>0.05),但较低剂量组明显改善(P<0.05)。三个实验组c-sis基因的差异无显著性(P>0.05)。结论:GBE可以有效防止PTA后再狭窄,其机制可能与拮抗PAF、清除自由基、降低血清脂质水平以及抑制c-sis基因和c-myc蛋白表达有关。

Study of Ginko Biloba Extraet(GBE) on Prevention of Restenosis after Percutaneous Transluminal Angioplusty (PTA)

Objective:To observe the effects of Ginko biloba extract(GBE)on prevention of restenosis and investigate its mechanism.Method:After establishing the model of artheroscle-rosis stenosis,40 rabbits were randomly divided into four groups:model group(n=10)received percutaneous transluminal angioplasty(PTA),and low-dose group(n=10),medium-dose group(n=10)and high-dose group(n=10)received PTA and GBE 0.16 g/kg/d,0.32 g/kg/d,0.48 g/kg/d,respectively,and control group(n=10)only received normal food.Low density lipoprotein-cholesterol(LDL-C),high density lipoprotein-cholesterol(HDL-C),total cholesterol(TC),platelet activating factor(PAF),superoxide dismutase(SOD),lactoperoxidase(LPO),malondialdehyde(MDA),c-sis gene and c-myc protein were examined respectively.Results:Compared to the control group,all of the characters in model group became worse.But compared to the model group,LDL-C,TC,LPO,MDA,PAF,c-sis gene and c-myc protein decreased significantly and HDL-C,SOD increased significantly in the treatment group(P<0.05).LDL-C,TC,LPO,MDA,PAF,HDL-C,SOD,and c-myc protein had not significant differences between the middle and high dose group(P>0.05),but better than low dose group(P<0.05).There was no significant difference between the three treatment groups in c-sis gene(P>0.05).Conclusion:GBE could prevent restenosis and it may be related to rivalry of PAF,to clean up free radical,to decrease serum lipoids and to inhibit the expression of c-sis gene and c-myc gene.