RF对纳米金标记的RNase-S局部加热效应的研究

目的 在纳米级或微米级结构的线度可以和热辐射波长λ相比拟的状态下的辐射热传导的研究具有重要的实用意义，通过建立生物模型来研究射频电磁辐射(RF)对纳米结构辐射热传导的特征，并探讨微波非热效应的可能作用机制。方法 将制备的纳米金标记的RNase-S样品通过常规的Poly-C水解能力的测定及哪谱的测定分析，确定酶的2级结构的重组成功和活性的存在后，比较RF对样品、水和空气的热效应。结果 通过紫外分光光度计对制备的纳米金标记的RNase-S样品的Poly-C水解能力的测定及谱的测定，表明研究中使用的生物模型和RNase A模拟得十分满意。空气浴中的封装空气样品的散热曲线呈现指数衰减规律，而空气样品的加热过程呈指数增加趋势。空气浴中的双蒸馏水样品的加热过程基本上是线性增加。但是，水浴的纳米金标记的RNase-S样品的加热过程却基本上保持恒定值。结论 RF辐射在实验的条件下会由于被加热介质的不同热物理性质表现为不同的加热效果，对于含有纳米金属的微结构，可能会在局部产生微涡流发热。以纳米颗粒为热源的辐射热传导可能会和生物大分子的结构调整发生谐振从而不表现出样品整体宏观的升温。生物功能性大分子关键部位局部加热的谐振吸收是微波等物理因素的非热生物效应的可能作用机制。

Local thermal effect of RF on nanogold-labeled Rnase

Objective To establish a biological model with nanostructure and to investigate the characteristics of the thermal conduction by radio frequency electromagnetic radiation upon the model for the exploration of the possible mechanism of microwave non thermal effect. Methods The prepared nanogold labeled RNase S sample was analyzed by routine hydrolytic ability of poly c and CD spectroscopy. After confirmation of the successful recombination of the enzymatic structure and the recovery of the enzyme activity, thermal effects of RF on the sample, water and air were compared. Results The biological model designed and prepared by us satisfactorily simulated the native RNase A in both recovery of enzyme activity and conformation. Air sample in air bathing possessed an exponent decreasing function for thermal dispersion and an exponent increasing function for RF thermal effect. The redistilled water sample had a linear increasing function for RF thermal effect. Meanwhile, nanogold labeled RNase S in water bathing kept constant for RF thermal effect. Conclusion Micro eddy current local heating might occur in microstructure consisting of nanometal cluster. A biological macromolecule with nanostructure, acting as a source of thermal radiation and conduction, might resonantly interact with some changeable domains nearby, so the biological model keeps isothermal during RF heating. This may be a possible mechanism of nonthermal effect for microwave and other physical factors.