| 氧化应激对人淋巴细胞端粒长度的影响 |
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| 引用本文： | 宗峰,丁国宪,程蕴琳. 氧化应激对人淋巴细胞端粒长度的影响[J]. 实用老年医学, 2005, 19(1): 25-27 |
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| 作者姓名： | 宗峰 丁国宪 程蕴琳 |
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| 作者单位： | 210029,江苏省南京市,南京医科大学第一附属医院老年科 |
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| 摘 要： | 目的 探讨人外周血淋巴细胞遭受过氧化氢 (H2O2 )损伤时,端粒长度的变化。 方法 体外培养人外周血淋巴细胞,分为正常对照组、氧化组和抗氧化组,然后应用Southern杂交技术检测不同时间端粒长度的变化。 结果 端粒长度随着时间的变化表现为缩短;且 3组之间均有显著差异(P<0 .05)。 结论 氧化应激在一定程度上可以引起人淋巴细胞端粒长度的缩短;维生素C具有一定的抗氧化作用,它能减低过氧化损伤,从而减缓端粒缩短的速率。
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| 关 键 词： | 氧化性应激 淋巴细胞 端粒 |
| 文章编号： | 1003-9198(2005)01-0025-03 |
| 修稿时间： | 2004-06-21 |
Variation of telomere length in human lymphocytes caused by oxidative stress |
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| ZONG Feng,DING Guo-xian,CHENG Yun-lin. Variation of telomere length in human lymphocytes caused by oxidative stress[J]. Practical Geriatrics, 2005, 19(1): 25-27 |
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| Authors: | ZONG Feng DING Guo-xian CHENG Yun-lin |
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| Affiliation: | ZONG Feng,DING Guo-xian,CHENG Yun-lin.Department of Geriatrics,the First Affiliated Hospital,Nanjing Medical University,Nanjing 210029 China |
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| Abstract: | Objective To observe the variation of telomere length of human peripheral blood lymphocytes caused by oxidative stress. Methods Human lymphoid cells were cultured in vitro and divided into three groups:oxidative,anti- oxidative using Vit.C and control group.Southern hybridizing was applied to determine telomere length. Results Telomere lengths varied with the elapse of time and three groups had significant difference(P<0.05). Conclusions Oxidative stress may cause telomere shortening to some extent,and Vit.C can slow the rate of telomere shortening. |
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| Keywords: | Oxidative stress Lymphocytes Telomere |
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