| 小肠缺血再灌注损伤导致Cajal间质细胞凋亡的实验研究 |
| |
| 引用本文： | 韩鹃,何扬涛,梅峰.小肠缺血再灌注损伤导致Cajal间质细胞凋亡的实验研究[J].中国局解手术学杂志,2010,19(2):80-82. |
| |
| 作者姓名： | 韩鹃 何扬涛 梅峰 |
| |
| 作者单位： | 第三军医大学基础医学部组织学与胚胎学教研室; |
| |
| 基金项目： | 国家自然基金资助项目(30900465);;重庆市自然科学基金资助项目(2009BB5157) |
| |
| 摘 要： | 目的探讨小肠缺血再灌注损伤对Cajal间质细胞（interstitial cells of Cajal,ICC）的影响。方法采用缺血60 min再灌注6 h（I60/R6h）、12 h（I60/R12h）和14 d（I60/R14d）的豚鼠小肠,通过免疫细胞化学和TUNEL法检测ICC的凋亡。结果I60/R6h豚鼠小肠可见少量TUNEL阳性ICC。I60/R12h可见较多的凋亡ICC,同时ICC细胞数量明显减少,细胞网络不完整。I60/R14d的ICC恢复正常数量和分布。不同时间点之间Kit/TUNEL双标阳性细胞数量存在显著差别（P〈0.05）。结论小肠缺血再灌注损伤导致ICC凋亡,一段时间后ICC能够恢复正常。
|
| 关 键 词： | Cajal间质细胞 凋亡 Kit蛋白 TUNEL |
A study on apoptosis of interstitial cells of Cajal induced by intestinal ischemia and reperfusion injury |
| |
| HAN Juan,HE Yang-tao,MEI Feng.A study on apoptosis of interstitial cells of Cajal induced by intestinal ischemia and reperfusion injury[J].Journal of Regional Anatomy and Operative Surgery,2010,19(2):80-82. |
| |
| Authors: | HAN Juan HE Yang-tao MEI Feng |
| |
| Institution: | Department of Histology and Embryology;Third Military Medical University;Chongqing 400038;China |
| |
| Abstract: | Objectives To investigate the apoptosis of interstitial cells of Cajal induced by intestinal ischemia and reperfusion injury.Methods The small intestine were resected at 6 hours(I60/R6h),12 hours(I60/R12h) or 14 days(I60/R14d) after 60 min intestinal isechemia and reperfusion in adult guinea pigs and studied by immunohistochemistry and TUNEL method.Results Kit/TUNEL double labeling cells were found out in the small intestine at I60/R6h and I60/R12h,and those ICC also decreased in cell number.ICC recovered t... |
| |
| Keywords: | interstitial cells of Cajal apoptosis Kit TUNEL |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
