中风安口服液对动脉血栓形成和血液凝固的干预效应

背景中风安口服液是治疗脑血管疾病的新型中药制剂,主要成分为黄芪和水蛭,其中黄芪具有明显的益气活血作用,水蛭具有很强的抗血小板、抗血栓、缓解动脉痉挛和改善组织缺氧的作用.目的观察中风安口服液对大鼠动脉血栓形成和对小鼠血液凝固及耐力的影响.设计完全随机对照实验.单位河北医科大学基础医学院药理教研室.材料实验于2001-09/2002-04在河北医科大学药理研究室完成.药物影响血栓形成实验第1组实验取Wistar大鼠40只,随机分为中风安3.0,6.0,12.0g/kg剂量组、阿司匹林0.3 g/kg组和对照组,每组8只.第2组实验取Wistar大鼠50只,随机分为中风安3.0,6.0 g/kg剂量组、脑血康3.0,6.0 g/kg剂量组和对照组,每组10只.小鼠凝血时间实验取小鼠50只,随机分为中风安6.0,12.0,24.0 g/kg剂量组、阿司匹林0.3 g/kg组和对照组,每组10只.小鼠游泳耗竭时间测定取小鼠90只,随机分为中风安6.0,24.0 g/kg剂量组;脑血康6.0,24.0 g/kg剂量组,苯丙胺0.2 g/kg组和对照组,每组15只.方法药物影响血栓形成实验,第1组于术前24 h及1 h各组大鼠分别灌胃给予中风安(3,6,12g/kg),阿司匹林(0.3g/kg)和水.第2组大鼠术前24h及1 h分别灌胃给予中风安(3,6g/kg)脑血康(3,6 g/kg)和水.给药后腹腔注射氯胺酮50 mg/kg麻醉,采用线栓血栓法,测定各鼠血栓湿重,比较各组血栓形成的差异.小鼠凝血时间的测定,分别灌胃给予中风安(24.0,12.0,6.0 g/kg),阿司匹林(0.3 g/kg)和水,于灌胃后1 h用玻片法观察小鼠凝血时间.小鼠游泳耗竭时间的测定,灌胃给予中风安(6.0,24.0 g/kg)和脑血康(6.0,24.0 g/kg)及苯丙胺(0.2 g/kg,)和水.1次/d,连续灌胃5 d,于第5天给药后1 h测定小鼠游泳耗竭时间.计算各组小鼠游泳耗竭时间的平均值.主要观察指标①各组大鼠血栓形成抑制率.②各组小鼠凝血时间.③各组小鼠游泳耗竭时间.结果参加实验的90只大鼠和140只小鼠均进入结果分析.①血栓湿重第1组实验中风安口服液3.0,6.0,12.0 g/kg剂量组明显低于对照组(24±4),(21±4),(16±6),(39±7)mg,(t=5.88～7.90,P＜0.01)];第2组实验中风安口服液6.0 g/kg剂量组明显低于相同剂量脑血康组(23.6±2.6),(30.0±4.1),(t=4.18,P＜0.01)],中风安口服液3.0 g/kg剂量组低于同剂量脑血康组(30.6±2.1),(33.1±1.6)mg,(t=2.96,P＜0.05)].②凝血时间中风安口服液24.0,12.0g/kg剂量组明显高于对照组(222±66),(190±52),(116±26)s,(t=4.02,4.72,P＜0.01)].③游泳耗竭时间中风安24.0,6.0g/kg剂量组明显高于对照组(2 512±1 244),(899±403),(502±100)s,(t=3.70～6.24,P＜0.01)].结论中风安口服液对大鼠动脉血栓和小鼠静脉血栓的形成均有明显的抑制作用,并可增强小鼠的耐力,具有抗疲劳作用.

Effect of oral zhongfeng an liquid on arterial thrombosis and blood coagulation

OBJECTIVE: Oral zhongfeng an liquid is a new dose form of traditional Chinese medicine for treating cerebrovascular diseases. Its main components are astragalus and hirudo, the former is of obvious effects of replenishing qi and activating blood, and the latter is of stronger effects of antiplatelet, antithrombosis and arteriospasm-reducing, as well as improving tissue anoxia.OBJECTIVE: To investigate the effects of oral zhongfeng an liquid on arterial thrombosis in rats, and blood coagulation and tolerance in mice.DESIGN: A completely randomized and controlled trial.SETTING: Pharmacological Division of Basic Medical College, Hebei Medical University.MATERIALS: The experiment was completed from September 2001 to April 2002 at the Pharmacological Division of Basic Medical College,Hebei Medical University. The experiment of effect of the drug on thrombosis in rats: In the first study, totally 40 Wistar rats were at random divided into five groups: zhongfeng an liquid 3.0, 6.0, 12.0 g/kg, aspirin 0.3 g/kg and control group, with 8 in each group. In the second study, totally 50 Wistar rats were also at random divided into five groups: zhongfeng an liquid 3.0 and 6.0 g/kg, naoxue kang 3.0 and 6.0 g/kg and control group,with 10 in each group. Clotting time study in mice: Totally 50 mice were randomly divided as zhongfeng an liquid 6.0, 12.0, 24.0 g/kg, aspirin 0.3 g/kg and control group, with 10 in each group. Measurement of swimming exhaustion time of mice: Totally 90 mice were randomly divided as zhongfeng an liquid 6.0 and 24.0 g/kg, naoxue kang 6.0 and 24.0 g/kg, benzedrine 0.2 g/kg and control group, with 15 in each group.METHODS: In the experiment of effect of the drug on thrombosis: For the first study, 24 hours and 1 hour before operation the rats in all groups were respectively by gavage given oral zhongfeng an liquid (3, 6, 12 g/kg), aspirin (0.3 g/kg), and water. For the second study, 24 hours and 1 hour before operation the rats were respectively by gavage given oral zhongfeng an liquid (3, 6 g/kg), naoxue kang (3, 6 g/kg) and water. After administration,ketamine 50 mg/kg was peritoneally given for anesthesia, silk ligature thrombosis was used, then the wet thrombus was weighed for comparison of difference in thrombosis among the groups. Measurement of clotting time of mice: The mice were respectively by gavage given oral zhongfeng an liquid (24.0, 12.0, 6.0 g/kg), aspirin (0.3 g/kg) and water, one hour after administration the clotting time of mice was detected with the slide method. Measurement of swimming exhaustion time of mice: The mice were respectively by gavage given oral zhongfeng an liquid (6.0, 24.0 g/kg), naoxue kang (6.0, 24.0 g/kg), Benzedrine (0.2 g/kg) and water, once a day for 5 days.On the fifth day 1 hour after administration, the swimming exhaustion time of mice was measured, the mean value of swimming exhaustion time of mice in each group was calculated.haustion time of mice in each group.RESULTS: All 90 rats and 140 mice involved entered into the result thrombus in rats of the oral zhongfeng an liquid 3.0, 6.0 and 12.0 g/kg groups were obviously lower than those in the naoxue kang groups of the same dose (24±4), (21±4), (16±6), (39±7) mg, (t=5.88-7.90, P ＜ 0.01)]; in the second study, the wet quality of rats in the oral zhongfeng an liquid 6.0 g/kg group was obviously lower than that in the same dose naoxue kang group (23.6±2.6), (30.0±4.1), (t=4.18, P ＜ 0.01)], the wet quality of mice in the oral zhongfeng an liquid 3.0 g/kg group was obviously lower than that in the same dose naoxue kang group (30.6±2.1), (33.1±1.6) mg, (t=2.96,zhongfeng an liquid 24.0 and 12.0 g/kg groups were obviously higher than that in the control group (222±66), (190±52), (116±26) s, (t=4.02, 4.72, P the oral zhongfeng an liquid 24.0 and 6.0 g/kg groups were obviously higher than that in the control group (2 512±1 244), (899±403), (502±100) s,(t=3.70-6.24, P ＜ 0.01)].CONCLUSION: Oral zhongfeng an liquid was of obvious inhibition to arterial thrombosis of rats and venous thrombosis of mice, and could enhance the tolerance of mice with a role of antifatigue.