牛奶菜化学成分的研究

目的: 追踪分离牛奶菜的化学成分，并对分离得到的单体化合物进行抗肿瘤活性测试。方法: 以体积分数70%乙醇回流提取，采用各种色谱技术对提取物进行分离纯化，通过理化性质和光谱数据确定所得单体化合物的结构。结果: 从牛奶菜根的提取物中分离并鉴定了10个化合物，分别鉴定为α-香树脂醇乙酸酯 (1)、α-香树脂醇 (2)、3β, 24-二羟基乌苏烷-12-烯 (3)、补骨脂素 (4)、β-胡萝卜苷 (5)、异香草酸 (6)、α-单棕榈精 (7)、亚油酸 (8)、二十四烷酸 (9)、十六烷醇 (10)。结论:化合物3、4、6、7为首次从该植物中分离得到。

Chemical constituents of radix Marsdeniae sinensis

Aim To separate and determine the chemical constituents of radix Marsdeniae sinensis,and evaluate their antiproliferative activities against human tumor cells.Methods Extracted with 70% ethanol,the extract was isolated and purified by means of various chromatographic techniques,the structures of pure compounds were elucidated based on their physico-chemical properties and spectroscopic data.Results 10 Compounds were isolated and their structures were identified as α-amyrin acetate（1）,α-amyrin（2）,3β,24-dihydroxyurs-12-ene（3）,psoralen（4）,β-daucosterol（5）,isovanillic acid（6）,palmitic monoglycerol ester（7）,cis-9,cis-12-octadecadienoic acid（8）,lignoceric acid（9）,and 1-hexadecanol（10）,respectively.Conclusion Compounds 3,4,6 and 7 were isolated for the first time from the plant.