Polypeptide composition of normal and neoplastic human breast tissues and cells analyzed by two-dimensional gel electrophoresis |
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Authors: | Terry M Maloney Philip L Paine Jose Russo |
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Institution: | (1) Department of Research Administration, Michigan Cancer Foundation, 110 E. Warren Avenue, 48201 Detroit, MI, USA;(2) Department of Biological Sciences, St. John's University, 11439 Jamaica, New York, USA;(3) Department of Pathology, Michigan Cancer Foundation, 110 E. Warren Avenue, 48201 Detroit, Michigan, USA |
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Abstract: | Summary The protein populations of epithelial cells cultured from two neoplastic and five non-neoplastic human breast tissues were resolved and displayed by two-dimensional polyacrylamide gel electrophoresis and silverstaining. With a computer-based image analysis system, we identified eight polypeptides which are present in both of the neoplastic cell lines, but absent from all five of the cultures of non-neoplastic breast cells. The eight polypeptides are not unique to cells cultured from neoplastic breast, because they are also found in cells cultured from non-breast tissues, both neoplastic and non-neoplastic. Two of the eight polypeptides ( Mr 25,000/pI 4.4 and Mr 31,000/pI 5.5) are present in the patterns of whole tissue samples from infiltrating ductal carcinomas and absent in most normal breast tissue. |
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Keywords: | breast cancer cell lines image analysis infiltrating ductal carcinoma polypeptides two-dimensional polyacrylamide gel electrophoresis |
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