T regulatory markers expression in unexplained recurrent spontaneous abortion |
| |
| Authors: | Bita Saifi Reza Aflatoonian Nader Tajik Mahmood Erfanian Ahmadpour Rosita Vakili Fatemehsadat Amjadi |
| |
| Affiliation: | 1. Department of Anatomical Sciences, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran,;2. Department of Anatomical Sciences, Mashhad Medical Science Branch, Islamic Azad University, Mashhad, Iran,;3. Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran,;4. Department of Immunology, Faculty of Medicine, Division of Immunogenetics, Iran University of Medical Sciences, Tehran, Iran,;5. Department of Anatomical Sciences, Mashhad Medical Science Branch, Islamic Azad University, Mashhad, Iran,;6. Center of Pathological and Medical Diagnostic Services, Iranian Academic Center for Education, Culture &7. Research (ACECR), Mashhad Branch, Mashhad, Iran,;8. Department of Anatomical Sciences, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran, |
| |
| Abstract: | Objective: To evaluate expression of glucocorticoid-induced tumor necrosis factor receptor (GITR), cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and IL-10 in peripheral blood mononuclear cells (PBMCs) of 20 women with unexplained recurrent spontaneous abortion (URSA) compared to 20 normal non-pregnant women (NNP) during luteal phase in the window of implantation.Methods: Quantitative real-time PCR (qRT-PCR) was performed using the Taqman method for expression of GITR and SYBR Green method for expression of CTLA-4 and IL-10.Results: Expression of CTLA-4 in the NNPs (median; interquartile range; 3; 1.8–10) was significantly higher than the URSAs (0.72; 0.26–3.81, p?=?0.015). Expression of GITR in the NNPs (53; 10–139) was significantly higher than the URSAs (6; 3–27, p?=?0.005). However, IL-10 expression in the URSAs was significantly higher than the NNPs, did not meet a significant value. A significant correlation was found between CTLA-4 and GITR expression in the study population (p?=?0.0001).Conclusions: Expression of CTLA-4 and GITR were significantly down-regulated in the URSAs compared to NNPs at the window of implantation, which shows the essential role of Treg cells in creating an immunological privileged site for fetus as an allograft at the maternal–fetal interface by high expression levels of CTLA-4 and GITR during a normal pregnancy. |
| |
| Keywords: | CTLA-4 GITR IL-10 unexplained recurrent spontaneous abortion |
|
|
