Geraniin induces apoptosis of human breast cancer cells MCF-7 via ROS-mediated stimulation of p38 MAPK |
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| Authors: | Jia-Wen Zhai Chang Gao Wei-Dong Ma Wei Wang Li-Ping Yao Xin-Xin Xia |
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| Affiliation: | 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, PR China,;2. Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin, PR China,;3. Collaborative Innovation Center for Development and Utilization of Forest Resources, Northeast Forestry University, Harbin, PR China,;4. Peking University People’s Hospital, Beijing, PR China |
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| Abstract: | Geraniin, a typical ellagitannin isolated from Phyllanthus urinaria Linn, has been found to possess a range of bioactive properties. In the present study, we found that Geraniin showed potent anti-proliferative effects on human breast cancer MCF-7 cells. The IC50 values were 9.94, 17.98 and 42.32?µM after 72-, 48- and 24-h treatment, respectively. Meanwhile, Geraniin could remarkably disrupt mitochondrial membrane potential and arrest S phase cell cycle. Western-blot analysis showed that Geraniin induced phosphorylation of the anti-apoptotic Bcl-2, and the cleavage of poly (ADP-ribose) polymerase (PARP) and caspase-3 in MCF-7 cells. Moreover, Geraniin treatment activated p38 mitogen-activated protein kinase (p38 MAPK) and the effect was blunted in MCF-7 cells with the treatment of a specific p38 inhibitor SB203580. Geraniin could generate intracellular reactive oxygen species (ROS), activate p38 MAPK then induce the apoptosis in MCF-7 cells, such phenomena was abrogated by pretreatment with N-acetyl-l-cysteine. In general, these results support the conclusion that Geraniin-induced apoptosis is mediated via ROS-mediated stimulation of p38 MAPK signaling. |
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| Keywords: | Apoptosis geraniin MCF-7 cells mitochondrial p38 MAPK ROS |
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