In vitro inhibitory effects of thymol and carvacrol on dendritic cell activation and function |
| |
| Authors: | Zahra Amirghofran Hossein Ahmadi Mohammad Hossein Karimi Fathollah Kalantar Nasser Gholijani Zahra Malek-Hosseini |
| |
| Affiliation: | 1. Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran,;2. Autoimmune Diseases Research Center and Medicinal Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, amirghz@sums.ac.ir;4. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, and;5. Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;6. Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran, |
| |
| Abstract: | Context: Thyme has been used in traditional medicine for medicinal purposes since ancient times.Objective: The objective of this study was to investigate the effects of thymol and carvacrol as two major constituents of thyme on dendritic cells (DCs) maturation and T cell activation.Materials and methods: Splenic DCs were treated with non-cytotoxic concentrations of the components and then analyzed for MHC II, CD86, and CD40 expression by flow cytometry. The effects of compounds on mitogenic, as well as allogenic T cell responses in mixed lymphocyte culture (MLR) and the release of cytokines were investigated.Results: At 0.1?µg/ml, reduced mean fluorescent intensity (MFI) of CD86 for thymol (80.3?±?0.2% of untreated control) and CD40 for carvacrol (79.5?±?0.14%) was observed (p?p?p?p?p?p?Conclusion: These data showed the suppressive effects of thymol and carvacrol on DCs maturation and function, as well as T cell responses. |
| |
| Keywords: | CD40 CD86 immunomodulation interferon-γ interleukin-4 lymphocyte proliferation medicinal plants mixed lymphocyte culture |
|
|
