Immunomodulatory effects of Santolina chamaecyparissus leaf extracts on human neutrophil functions |
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| Authors: | Chahra Boudoukha Eduardo Ortega Abderrahmane Senator |
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| Affiliation: | 1. Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University of Ferhat Abbas, Setif, Algeria and;2. Group of Immunophysiology, Department of Physiology, Faculty of Sciences, University of Extremadura, Badajoz, Spain |
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| Abstract: | Context: Santolina chamaecyparissus L. (Asteraceae) is an aromatic plant wide spread in the Mediterranean region. It is used in folk medicine for its anti-inflammatory properties.Objective: The effects of S. chamaecyparissus aqueous extract (SCAE) and polyphenolic extract (SCPE) on human polymorphonuclear neutrophil (PMN) degranulation, chemotaxis, phagocytosis, and microbicidal capacity were examined in vitro.Materials and methods: Aqueous and polyphenolic extracts were prepared from S. chamaecyparissus leaves. The elastase release was used as a marker for measuring PMN degranulation, while chemotaxis was performed using a 48-microwell chemotaxis chamber. The phagocytosis and the microbicidal capacity were evaluated using fresh cultures of Candida albicans.Results: The treatment of neutrophils with different concentrations (10–200?µg/ml) of SCAE and SCPE caused a significant (p?p?Candida albicans was observed when PMNs were exposed to 100 and 200?µg/ml of SCAE or SCPE.Conclusion: The exerted effects on neutrophil functions support the anti-inflammatory activity and show new mechanisms of action and effectiveness of S. chamaecyparissus leaf extracts. This plant may be considered as an interesting source of anti-inflammatory and immunomodulatory agents. |
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| Keywords: | Chemotaxis degranulation inflammation phagocytosis plant extracts polyphenols |
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