PDGF-B基因转染骨髓间充质干细胞的研究

目的：探讨血小板源性生长因子B（PDGF-B）基因转染修饰大鼠骨髓闻克质干细胞（MSC）的可行性。方法：应用FAM标记重组真核表达载体系统（pcDNA3-PDGF-B）。以脂质体法转染原代MSC。观察转染结果、表达情况孕对靶细胞活力的影响。结果：MSC的基因转染成功，持续表达时间超过了8周，没有发现明显的细胞毒作用及对细胞活力的显著影响。结论：采用真柱转染技术可以介导外源基因转染MSC，MSC是一种理想的基因载体细胞。可用于PDGF-B的基因治疗。

Study on transfecting platelet-derived growth factor-B gene to bone marrow mesenchymal stem cells

Objective: To study the feasibility of transfecting platelet-derived growth factor-B(PDGF-B) gene to bone marrow mesenchymal stem cells(MSCs).Methods: Recombinant eukaryotic expression carrier system(pcDNA_(3)-PDGF-B) was marked with carboxyfluorescein(FAM).Original MSCs were transfected by using liposome technique.The results of transfection and the activity of the target cell were determined.Results: Bone marrow MSCs were successfully transfected with pcDNA_(3)-PDGF-B.The expression of exogenous gene continued over 8 weeks.No cell toxicity and obvious influence on cell activity was found.Conclusion: MSCs can be efficiently transfected with exogenous gene by using eukaryotic transfection technique.MSCs are ideal cells for cell-mediated transfection and can be used in PDGF-B gene therapy.