广州地区首次参加无偿献血人群HCV感染的流行病学调查

目的了解广州地区首次参加无偿献血人群HCV的感染状况。方法抗-HCV检测采用酶联免疫吸附实验(使用2种不同厂家的试剂盒),HCV RNA检测采用荧光定量PCR法,ALT检测采用速率法。结果2004—2007年在广州血液中心首次参加无偿献血的559890名献血者中,1617名经双试剂检测抗-HCV阳性,阳性率为0.289%。随机选取435名双试剂检测抗-HCV阳性血液标本进行RNA检测,266名阳性,占61.15%,男女(P<0.01)HCV RNA阳性率有统计学意义(P<0.01)。266名抗-HCV检测阳性献血者中,ALT升高(>40U)的为18名(其中男性17名),不合率为6.77%,男女ALT的不合格比有统计学意义(P<0.01)。结论随着无偿献血的开展,献血人群中HCV的阳性率不断下降;不同性别的个体清除HCV的能力不同,女性高于男性。

Seroepidemiological studies of HCV among first-time volunteer blood donors in Guangzhou,China

Objective To investigate the prevalence of HCV infection among first.time volunteer blood donors recruited during 2004-2007 in Guangzhou.Methods Anti-HCV was detected using two different third-generation EIA kits.HCV RNA was detected using fluorescent quantitat;on polymerase chain reaction(O-PCR).ALT was detected by the speed rate method.ResulIs A total of 1 617(0.289%)were positive for anti-HCV with two kits.Among them,435 were selected randomly for HCV nucleic acid amplification by Q-PCR,and 266(61.15%)were HCV RNA(+).The HCV RNA(+) rate was significantly higher in males than in females(66.89% vs 49.30%,P<0.01).Among the 266 HCV RNA(+) donors,18 showed abnormal ALT level(>40 U).The abnormal ALT rate was higher in males than female8(8.67% vs 1.43%),without any statistical difference(P>0.05).Conclusion The anti.HCV(+) rate among blood donors in Chi-na reflects a steady decreasing tendency,owing to the current recruitment of exclusively volunteer donors.Spontaneous clearance of HCV was more likely to occur in femalcs than males.