多种化疗药对凋亡抑制蛋白Survivin在HL-60细胞中表达影响的研究

Objective： To explore the relationship between survivin and drug resistance, and the changes of the survivin expression in HL-60 cells treated with three kinds of chemotherapeutic drugs. Methods： HL- 60 cells were treated with appropriate concentration of daunomycin （DNR）, mitoxantrone （MIT） or arsenic trioxide （As2O3）. The expression of survivin mRNA and protein on the first or third day was detected by RT-PCR and Western blot respectively. Results： The expression of survivin mRNA was decreased on the first day by 10% in DNR-treated group, 40% in MIT-treated group （P〈0.01） and 25% in As2O3-treated group （P〈0.01） respectively. On the third day, the expression of survivin mRNA in DNR- and MIT-treated group was up-regulated to 120% （P〈0.05） and 165% （P〈0.01） respectively as compared with that on the first day, but down-regulated to 68% in As2O3-treated group （P〈0.01）. As compared with control group, the expression of survivin protein in DNR- or MIT-treated group was increased by 14% or 11% on the third day respectively, but it was decreased by 18% in As2O3-treated group. Conclusion： In DNR- and MIT-treated group, the expression of surivin was decreased at first and then increased obviously, which may be one of the causes for resistance to chemotherapy against leukemia. Different from other two drugs, As2O3 may play an important role in restoring chemotherapy sensitivity.

Expression of survivin in HL-60 cells treated with chemotherapeutic drugs

Objective: To explore the relationship between survivin and drug resistance, and the changes of the survivin expression in HL-60 cells treated with three kinds of chemotherapeutic drugs. Methods: HL 60 cells were treated with appropriate concentration of daunomycin (DNR), mitoxantrone (MIT) or arsenic trioxide (As2O3). The expression of survivin mRNA and protein on the first or third day was detected by RT-PCR and Western blot respectively. Results: The expression of survivin mRNA was decreased on the first day by 10% in DNR-treated group, 40% in MIT-treated group (P<0.01) and 25% in As2O3-treated group (P<0.01) respectively. On the third day, the expression of survivin mRNA in DNR- and MIT-treated group was up-regulated to 120% (P<0.05) and 165% (P<0.01) respectively as compared with that on the first day, but down-regulated to 68% in As2O3-treated group (P<0.01). As compared with control group, the expression of survivin protein in DNR- or MIT-treated group was increased by 14% or 11% on the third day respectively, but it was decreased by 18% in As2O3-treated group. Conclusion: In DNR- and MIT-treated group, the expression of surivin was decreased at first and then increased obviously, which may be one of the causes for resistance to chemotherapy against leukemia. Different from other two drugs, As2O3 may play an important role in restoring chemotherapy sensitivity.