肺癌患者外周血血小板活化标志物水平及其临床意义

背景与目的 中、晚期肿瘤患者的血小板往往处于活化状态,其血小板黏附分子的表达和释放增加.本研究的目的是探讨肺癌患者手术前、后外周血血小板活化标志物的动态变化规律及其与肺癌的关系.方法 采用流式细胞术(flow cytometry,FCM)检测120例肺癌患者手术前、后外周血血小板活化标志物(CD62P和CD63)水平,以60例健康体检者作为对照.结果 肺癌患者手术前外周血CD62P、CD63水平均显著高于正常对照组(P＜0.01).肺癌患者术后第7天外周血CD62P、CD63水平显著低于术前和术后第1天(P＜0.01).肺癌患者术前外周血CD62P、CD63水平与肺癌原发肿瘤大小、有无淋巴结转移和分期有密切关系(P＜0.01),与肺癌细胞分化程度、组织学类型、患者性别和年龄无明显关系(P＞0.05).结论 肺癌患者外周血血小板活化标志物水平升高,且与肺癌的发生、发展和淋巴结转移有一定关系,因而有可能作为动态监测肺癌患者病情进展、判断预后的参考指标.

Levels of activation markers of platelets in peripheral blood and their significance in patients with lung cancer

Background and objective Platelet activation often occurs in intermediate and advanced tumors, with increases of expression and release of platelet adhesion molecule. The aim of this study is to investigate the expression of activation markers of platelet and their significance in lung cancer. Methods The activation markers of platelet, CD62P and CD63, were detected in peripheral blood of 120 patients with lung cancer and 60 healthy persons by FCM method. Results The levels of peripheral blood CD62P and CD63 of lung cancer patients were significantly higher than those of healthy people (P<0.01). In lung cancer group, the levels of peripheral blood CD62P and CD63 on the seventh postoperative day were significantly lower than those before operation and on the first postoperative day (P<0.01). The levels of peripheral blood CD62P and CD63 before operation were closely related to size of tumor, lymph node status and TNM stages (P<0.01), but not to cell differentiation, histology, age and sex of lung cancer patients (P>0.05). Conclusion Activation markers of platelet obviously increase in peripheral blood of lung cancer patients and they may play important roles in tumor growth and lymphatic metastasis. The levels of activation markers of platelet may be useful predictors for prognosis.