单纯疱疹病毒Ⅰ型潜伏感染的研究：兔角膜HSV—1抗原的检测

通过使用HSV-1 Mckrae株在角膜上建立HSK感染的动物模型,应用辣根过氧化物酶免疫组织化学染色技术检测HSV-1抗原在角膜组织内存在的时间,结果发现,在角膜病变消失后5、10、20、30、45天的角膜基质组织内均有HSV-1抗原存在,但在60、80天的切片上抗原阴性。表明HSV-1原发感染消失后抗原在角膜基质内存在45天后转为阴性。说明角膜基质细胞是对HSV-1感染的最敏感细胞。

Investigation of HSV-1 Latent Infection in Rabbit Cornea: The Presence of HSV-1 Antigen in Cornea after Primary Infection

Through theinoculationof HSV-1 Mckrae strain in the cornea, we established the experimental animal model of primary herpes simplex keratitis (HSK). With the technique of horseradish peroxidase immunohistochemistry staining, we detected the presence of HSV-1 antigens in the corneal tissue and found that HSV-1 antigens were present for 45 days after the corneal lesion disappeared; in corneal sections of 60, 80 days, no antigens were found. The results, which HSV-1 antigens became negative in corneal tissue at 45 days after primary infection has gone, showed that the kerotocytes are the most sensitive cells to HSV-1 infection and inferred that the cornea maybe become another site of HSV-1 latency. The studies will also be of the important significance to the illustration of clinical signs and the direction of therapy of HSK.