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抗肿瘤中药对小鼠U14宫颈癌细胞多种酶活性的影响
引用本文:赵迅霞,芦晓红,贾月霞,曹军. 抗肿瘤中药对小鼠U14宫颈癌细胞多种酶活性的影响[J]. 宁夏医科大学学报, 2002, 24(6): 403-405
作者姓名:赵迅霞  芦晓红  贾月霞  曹军
作者单位:[1]宁夏医学院机能学科实验中心,银川750004 [2]宁夏医学院生命学科实验中心,银川750004
摘    要:目的:探讨抗肿瘤中药对癌细胞的繁殖起重要作用的ACPase、ATKPase、SDH、G-6-PDH在U14宫颈癌细胞中活性的影响。方法:荷瘤小鼠在第7d用药后6h处死,取腹水癌细胞涂片,固定显示,用细胞分光光度计测定酶活性。结果:中药合剂实验组细胞内ACPase、ATPase、SDH、G-6-PDH酶活性比生理盐水对照组显著降低(P<0.01),分别下降60%、40%、56%、70%,与环磷酰胺组比无显著性差异(P>0.05)。结论:抗肿瘤中药合剂能显著地抑制U14宫颈癌细胞内ACPase、ATPase、SDH、G-6-PDH活性,呈现抑瘤作用。

关 键 词:抗肿瘤中药 小鼠 U14 宫颈癌 酶活性 细胞化学
文章编号:1005-8486(2002)06-0403-03
修稿时间:2002-09-04

Effect of Antitumor Chinese Medicine on Activities of ATPase, ACPase, SDH,G-6-PDH in U14 Cervix Cancer Cells
ZHAO Xun-xia,LU Xiao-hong,JIA Yue-xia,et al. Effect of Antitumor Chinese Medicine on Activities of ATPase, ACPase, SDH,G-6-PDH in U14 Cervix Cancer Cells[J]. Journal of Ningxia Medical College, 2002, 24(6): 403-405
Authors:ZHAO Xun-xia  LU Xiao-hong  JIA Yue-xia  et al
Abstract:Objective: To test the effect of antitumor Chinese medicine on activities of ATPase, ACPase, SDH,G-6-PDH in U 14 cervix cancer cells, those enzymes are crucial for cancer cell proliferation. Methods: Mice with U 14 ascites carcinoma were killed by neck luxation, U 14 cells in ascites were aspitrated out, smeared on slices, fixed, color-developed according to the instruction of kits, color-measured by using cytospectrophotometer and intracelluar enzymatic activities were eventurally resulted via statistical calculation. Results: The activities of ATPase, ACPase, SDH,G-6-PDH were significantly decreased (P<0 01) by 60%, 40%,56%,and 70% respectively, compared with normal control group. The difference between Chinese medicine group and cyclophosphamide group showed no significance (P>0 05). Conclusion: The antitumor Chinese medicine can significantly inhibite the activites of ATPase, ACPase, SDH,G-6-PDH in U 14 cervix cancer cells,thus exert a powerful suppression action on tumor cells and reasonably be an ideal antitumor medicine.
Keywords:antitumor Chinese medicine  tumor  cytochemistry  enzyme  activity
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