小鼠胚胎干细胞向成牙本质样细胞的诱导分化

目的探讨胚胎干细胞(ES细胞)向成牙本质样细胞诱导分化的方法。方法首先通过悬浮培养的方式,将ES细胞诱导分化形成拟胚体,然后将拟胚体细胞与牙髓成纤维细胞通过Transwell培养系统共培养,研究拟胚体细胞向成牙本质样细胞的分化情况。结果RT-PCR结果显示,拟胚体细胞与牙髓成纤维细胞共培养10d后,可检测到成牙本质细胞的特征性分子——牙本质涎磷蛋白(DSPP)的表达;共培养15d后,表达有所增强。而单独培养的拟胚体细胞,则始终未检测到DSPP的表达。结论通过与牙髓成纤维细胞共培养,可以促进胚胎干细胞向成牙本质样细胞分化。

Induction of mouse embryonic stem cells forming odontoblast-like cells by co-culture with pulp fibroblast

PURPOSE: To investigate the methods of induction of mouse embryonic stem cells to differentiate into odontoblast-like cells by co-culture with pulp fibroblast. METHODS: By suspension culture, embryonic stem cells were induced to form embryoid bodies. Then the cells from embryoid bodies were co-cultured with pulp fibroblast in Transwell system for differentiation toward odontoblast-like cells. RESULTS: By RT-PCR analysis, embryoid body cells gave rise to the cell population expressing DSPP, a specific odontoblast cell marker, after 10 days of co-culture with pulp fibroblast and the expression of DSPP was enhanced after 15 days of co-culture. On the other hand, the expression of DSPP was not detected in the isolately cultured embryoid body cells. CONCLUSIONS: Embryonic stem cells can be induced into odontoblast-like cells by co-culture with pulp fibroblast. Supported by Science and Technology Development Fund of Shanghai Municipality (Grant No. 03ZR14027).