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| 地黄叶片器官形态建成的研究 | |
| 引用本文： | 薛建平,王汝毅,张爱民,柳俊.地黄叶片器官形态建成的研究[J].中国中药杂志,2003,28(1):21-24. |
| 作者姓名： | 薛建平 王汝毅 张爱民 柳俊 |
| 作者单位： | 1. 淮北煤炭师范学院,生物系,安徽,淮北,235000;华中农业大学,园艺林学学院,湖北,武汉,430070 2. 淮北煤炭师范学院,生物系,安徽,淮北,235000 3. 华中农业大学,园艺林学学院,湖北,武汉,430070 |
| 摘 要： | 目的 :研究地黄叶片器官发生的形态建成 ;建立诱导地黄叶片直接再生植株和试管小地黄的最佳培养基和培养条件。方法 :从无菌脱毒苗上采取不同位置的叶片 ,接种到附加不同激素的培养基中 ,诱导叶片直接再生植株和试管地黄。结果与结论 :MS +6 BA 2 .0mg·L-1培养基最适合叶片再生植株。MS +6 BA 1.0mg·L-1+NAA 0 .5mg·L-1培养基适合诱导试管地黄。最佳培养条件是光照 12h·d-1,光强 2 0 0 0～ 3 0 0 0lx ,温度 (2 5± 1)℃。 |
| 关 键 词： | 地黄 叶片 再生植株 试管地黄 |
| 文章编号： | 1001-5302(2003)01-0021-04 |
| 收稿时间： | 2002/6/21 0:00:00 |
| 修稿时间： | 2002年6月21日 |
Studies on Organ Phytomorphology of Leaveos from Rehmannia glutinosa |
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| XUE Jian-ping ;;WANG Ru-yi ;ZHANG Ai-min ;Liu Jun.Studies on Organ Phytomorphology of Leaveos from Rehmannia glutinosa[J].China Journal of Chinese Materia Medica,2003,28(1):21-24. | |
| Authors: | XUE Jian-ping ;;WANG Ru-yi ;ZHANG Ai-min ;Liu Jun |
| Institution: | Department of Biology, Huaibei Coal Industry Teacher's College, Huabei 235000, Anhui, China. xuejp2000@yahoo.com.cn |
| Abstract: | OBJECTIVE: To set up the fittest medium and optimum condition for leaves from Rehmannia glutinosa to form regenerated plantlets directly and to induce microtubers. METHOD: Leaves of different phases obtained from the virus-free plantlets were incubated in some kinds of media on the back side. RESULT AND CONCLUSION: The fittest medium for the formation of regenerated plantlet was MS + 6-BA 2.0 mg.L-1 and the fittest medium for the induction of microtuber was MS + 6-BA 1.0 mg.L-1 + NAA 0.5 mg.L-1. The optimum incubation temperaue was 25 degrees C and the light length was (2,000-3,000 lx) 12 h.d-1. |
| Keywords: | Rehmannia glutinosa leaf regenerated plantlet tuberous root |
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