Cyclin D1、p27在肺癌中表达的研究

目的研究Cyclin D1、p27基因蛋白在肺癌组织中的表达与肺癌临床病理特征的关系。方法：应用免疫组化S-P法检测109例手术切除肺癌组织标本中两种基因蛋白的表达。结暴：肺癌Cyclin D1基因过度表达的总阳性率为93.6%(102/109)。其中鳞癌为98%（50/51)，腺癌为97.2%（35/36），小细胞癌为77.3%(17/22);p27基因表达的阳性率为33%(36/109），其中麟癌为39.1%(20/51)，腺癌为25%(9/36），小细胞癌31.8%（7/22）。Cyclin D1阳性表达与肺癌的分化程度、TNM分期呈负相关。Cyclin D1和p27蛋白在肺癌组织中的表达呈负相关。结论：Cyclin D1和p27基因蛋白表达与肺癌发生、发展有关。

Study of Cyclin D1 and P27 Expressions in Lung Cancer

Objective To study the relationship between the Cyclin D 1 and P27 gene protein expressions and clinical pathological characteristics of lung cancer.Methods A sample of 109 lung cancer specimen was selected for the study. Two types of gene protein expressions were studied by using immunological S P measures for these specimens. Results The positive rate of the Cyclin D 1 over expression for lung cancer specimen was 93.6% (102 out of 109). Among them, squamous cell lung carcinomas was 98% (50 out of 51); adenocarcinomas 97.2 ( 35/36); small cell lung cancer 77.3 (17/22). For P27 gene, the overall rates were 33% (36/109). The respective rates were 39.1% (20/51), 25% (9/36), and 31.8% (7/22), for squamous cell lung carcinomas, adenocarcinomas and small cell lung cancer. There was a negative association between Cyclin D 1 positive expression and the degree of lung cancer differentiation. The association between Cyclin D 1 positive expression and the TNM stages of lung cancer was also negative. There was a negative relationship between Cyclin D 1 and P27 protein expressions.Conclusion The expressions of Cycin D 1 and P27 gene protein are related to the ontogenesis and progression of lung cancer.