慢性饮酒对大鼠胃粘膜细胞的凋亡与增殖的影响

目的 :研究大鼠慢性饮酒过程中胃粘膜的细胞凋亡和细胞增殖变化的规律 ,探讨慢性酒精刺激对胃粘膜的自我保护作用的影响。方法 :以 6% (V/V)的酒精作为大鼠饮水的唯一来源 ,利用流式细胞仪定量分析大鼠饮酒的不同时程内胃粘膜细胞的细胞周期和细胞凋亡 ,同时用免疫组化方法定性观察了大鼠胃粘膜的细胞增殖和凋亡情况。结果 :饮酒 3～ 2 8天的大鼠胃粘膜的细胞凋亡较对照组明显增加 ,在饮酒 3～ 14天的大鼠胃粘膜其细胞增殖也明显增加 ,提示在此时期内胃粘膜的细胞更新增强。结论 :长期低浓度酒精反复刺激可以促进胃粘膜的细胞凋亡并引起大鼠胃粘膜的自我保护作用减弱

Apoptosis and cell proliferation of the gastric mucosa in chronic alcohol drinking rats

Objective:To investigate the apoptosis and cell proliferation effect in the gastric mucosa of the chronic alcohol driking rats,and the effect of chronic ethanol exposures on the cytoprotection of the rats gastric mucosa.Methods:SD rats received the drinking water containing 6%(V/V) ethanol for 28 days.The cell cycle and apoptosis in gastric mucosa were analyzed by flow cytometer.To confirm our flow cytometric results,we also investigated the cell proliferation and apoptosis by immunohistochemistry.Results:The cell apoptosis in gastric mucosa of the rats were enhanced during they exposure to the ethanol in 3rd to 28th days.Otherwise the cell proliferation was increased in the period of 3 to 14 days.Conclusion:Chronic stimulus with the low concentration ethanol may enhance the cell apoptosis and reduced the cytoprotection of the rat gastric mucosa.