改良五黄油对成纤维细胞生长与增殖的影响

背景：烧伤创面外用药五黄油具有抑菌作用，能促进创面愈合；但在抑制瘢痕形成等方面疗效不确切。目的：观察改良五黄油不同药物浓度和药物作用时间对人成纤维细胞体外生长增殖的影响。设计、时间及地点：对比观察，细胞学体外实验于2006-04/2007-01 在南昌大学第一附属医院烧伤研究所完成。材料：包皮来自南昌大学第一附属医院和江西省儿童医院泌尿外科行包皮环切术的患者，年龄在2~12岁。患者家属均签署知情同意书。五黄油、改良五黄油复方制剂工作液(水溶性)由南昌大学第一附属医院药剂科提供。方法：体外培养人成纤维细胞。分为实验组和对照组。实验组加入300 g/L改良五黄油工作液；对照组加入300 g/L五黄油工作液。两组分别用无血清培养液配制成为6个质量浓度：0 g/L(空白对照组)，100，150，200，250，300 g/L。主要观察指标：于2，3，4，5，6 d，以MTT法检测细胞生长增殖情况；于2，4，6，8，10 d观察细胞生长抑制率。结果：以0~300 g/L质量浓度药物作用人成纤维细胞，其增殖抑制程度与改良五黄油的浓度存在正相关性；其抑制程度与培养时间无相关性。实验组与对照组相比，改良五黄油质量浓度在300 g/L时，8~10 d对成纤维细胞的抑制率更强，两组之间差异有显著性意义(P < 0.01)。结论：在一定的体外作用时间范围内，改良五黄油对体外成纤维细胞的增殖有一定抑制作用，并且抑制增殖的能力与药物浓度呈正相关。与五黄油相比较，300 g/L改良五黄油对体外成纤维细胞的生长抑制，有更好的效果。

Effect of modified Wuhuang oil on fibroblast growth and proliferation

BACKGROUND: Wuhuang oil has a bacteriostatic action to treat burn wound and promote traumatic healing, but the action on inhibition of scars formation remains poorly understood. OBJECTIVE: To investigate the effects of modified Wuhuang oil at different concentrations and administration times on the growth and proliferation of human fibroblasts in vitro.DESIGN, TIME AND SETTING: Comparison observation regarding cytology in vitro was performed at the Burns Institute in the First Affiliated Hospital of Nanchang University between April 2006 and January 2007.MATERIALS: Prepuce specimens were harvested from patients who underwent circumcision in Department of urinary surgery, at First Affiliated Hospital of Nanchang University and Jiangxi Provincial Children Hospital. All patients aged 2-12 years old, and informed consents were obtained from their relatives. Wuhuang oil and modified Wuhuang oil (water-solubility) were offered by Department of Pharmaceutical Preparation in the First Affiliated Hospital of Nanchang University, China. METHODS: Human fibroblasts cultured in vitro were divided into 2 groups at random, experiment and control. Experiment group was treated with 300 g/L Wuhuang oil, while control group with 300 g/L modified Wuhuang oil. Serum-free culture fluid was used to prepare 6 concentrations of oil solution: 0 (blank control), 100, 150, 200, 250, 300 g/L.MAIN OUTCOME MEASURES: MTT assay was used to determine the growth and proliferation of human fibroblasts at 2, 3, 4, 5, 6 days; inhibition rate of cell growth was observed at 2, 4, 6, 8, 10 days.RESULTS: Modified Wuhuang oil (0-300 g/L) concentration positively correlated with inhibition of human fibroblast proliferation; the inhibition was not related to culture time. Modified Wuhuang oil (300 g/L) had the greatest inhibition rate of human fibroblasts at 8-10 days, there were significant differences between experiment group and control group (P < 0.01). CONCLUSION: Modified Wuhuang oil has an effective inhibition on the proliferation of human fibroblasts in vitro, and shows a dose-dependent tendency. Compared with Wuhuang oil, 300 g/L modified Wuhuang oil is superior to suppress the growth of human fibroblasts.