| 神经酰胺诱导肝肿瘤Bel7402细胞凋亡(英文) |
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| 作者姓名： | Zhu XF Zhang XS Li ZM Yao YQ Xie BF Liu ZC Zeng YX |
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| 作者单位： | 中山医科大学肿瘤防治中心治疗基础室,中山医科大学肿瘤防治中心治疗基础室,中山医科大学肿瘤防治中心治疗基础室,中山医科大学肿瘤防治中心治疗基础室,中山医科大学肿瘤防治中心治疗基础室,中山医科大学肿瘤防治中心治疗基础室,中山医科大学肿瘤防治中心治疗基础室 广州 510060 中国,广州 510060 中国,广州 510060 中国,广州 510060 中国,广州 510060 中国,广州 510060 中国,广州 510060 中国 |
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| 摘 要： | 目的:了解神经酰胺信号转导在肝肿瘤细胞中的生物学意义。方法:MTT法、荧光染色、流式细胞术、琼脂糖凝胶电泳、Western blot。结果:神经酰胺对Bel7402细胞生长抑制的IC_(50)值为14.28μmol·L~(-1)。细胞核浓缩,荧光明显增强;DNA呈现典型“梯型”变化;p53蛋白表达呈下降趋势;Bcl-2蛋白表达明显降低;Bax蛋白表达无明显改变。结论:神经酰胺诱导肝肿瘤细胞凋亡,与Bcl-2表达下调有关。
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| 关 键 词： | 神经酰胺类 细胞凋亡 培养的肿瘤细胞 琼脂糖凝胶电泳 流式细胞术 荧光显微镜检查 蛋白质印迹 肝细胞癌 |
Apoptosis induced by ceramide in hepatocellular carcinoma Bel7402 cells |
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| Zhu XF,Zhang XS,Li ZM,Yao YQ,Xie BF,Liu ZC,Zeng YX.Apoptosis induced by ceramide in hepatocellular carcinoma Bel7402 cells[J].Acta Pharmacologica Sinica,2000,21(3):225-228. |
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| Authors: | Zhu X F Zhang X S Li Z M Yao Y Q Xie B F Liu Z C Zeng Y X |
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| Institution: | Department of Therapeutic Basis, Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. xfzhu@gzsums.edu.cn |
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| Abstract: | AIM: To study the biological function of ceramide signaling in Bel7402 cells. METHODS: Inhibition of cell growth was assayed using MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. DNA fragmentation was detected by electrophoresis and flow cytometry. The levels of protein p53, Bcl-2, and Bax were measured with Western blot. RESULTS: Bel7402 cells treated with C2-ceramide underwent cell proliferation inhibition. IC50 value was 14.28 mumol.L-1. After treatment of Bel7402 with ceramide, the morphologic changes including reduction in volume, nuclear chromatin condensation, fluorescence strength were observed. SubG1 peaks were detected on flow cytometry (FCM). Agarose gel electrophoresis of DNA from cells treated with ceramide revealed "ladder" pattern. The Western blot assay from cell extracts showed that the levels of protein p53 were decreased after ceramide treatment. The levels of protein Bcl-2 were decreased also. But the levels of Bax protein showed no difference between untreated cells and treated cells. CONCLUSION: Ceramide induces apoptosis in Bel7402 cells, related to Bcl-2 down-regulation. |
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| Keywords: | ceramides apoptosis cultured tumor cells agarose gel electrophoresis flow cytometry fluorescence microscopy Western blotting hepatocellularcarcinoma |
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