| 白果内酯刺激大鼠星形胶质细胞GDNF和VEGF表达(英文) |
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| 引用本文： | Zheng SX,Zhou LJ,Chen ZL,Yin ML,Zhu XZ. 白果内酯刺激大鼠星形胶质细胞GDNF和VEGF表达(英文)[J]. Acta pharmacologica Sinica, 2000, 21(2): 151-155 |
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| 作者姓名： | Zheng SX Zhou LJ Chen ZL Yin ML Zhu XZ |
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| 作者单位： | 中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所 上海 200031 中国,上海 200031 中国,上海 200031 中国,上海 200031 中国,上海 200031 中国 |
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| 基金项目： | Project supported by grants from Chinese Academy of Sciences (KJ952-J1-602 and KJ982-J1-614),Shanghai Science and Technology Commission (97XD14018). |
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| 摘 要： | 目的:观察白果内酯对大鼠星形胶质细胞GDNF和VEGF表达的影响。方法:应用半定量PCR了解大鼠星形胶质细胞分别经不同浓度白果内酯(0,5,15,50,100μmol·L~(-1))作用12、24和48h后细胞内GDNF和VEGF mRNA的变化,同时应用免疫组化了解经50μmol·L~(-1)作用24h后细胞内GDNF和VEGF蛋白的变化。结果:大鼠星形胶质细胞经50μmol·L~(-1)白果内酯作用12h后,细胞内GDNF和VEGF mRNA水平开始显著增高,经50μmol·L~(-1)作用24h后细胞胞浆内可见明显的VEGF和GDNF蛋白表达。结论:白果内酯可能通过诱导星形胶质细胞GDNF和VEGF的表达对神经细胞起保护作用。
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| 关 键 词： | 银杏 神经营养因子 星形胶质细胞 上调(生理学) 逆转录聚合酶链式反应 免疫组织化学 白果内酯 |
Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes |
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| Zheng S X,Zhou L J,Chen Z L,Yin M L,Zhu X Z. Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes[J]. Acta pharmacologica Sinica, 2000, 21(2): 151-155 |
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| Authors: | Zheng S X Zhou L J Chen Z L Yin M L Zhu X Z |
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| Affiliation: | Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | AIM: To study the effects of bilobalide on the expression of glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) in rat astrocytes in vitro. METHODS: Semiquantification polymerase chain reaction (SQ-PCR) was used to investigate GDNF and VEGF mRNA expression in the astrocytes after bilobalide (5, 15, 50, 100 mumol.L-1) treatment. Immunohistochemistry method was used to detect GDNF and VEGF protein expression in cells treated with bilobalide 50 mumol.L-1 for 24 h. RESULTS: GDNF and VEGF mRNA increased markedly after astrocytes were treated with bilobalide 50 mumol.L-1 for 12 h. GDNF and VEGF protein were detected in the cytoplasm of astrocytes after the cells were treated with bilobalide 50 mumol.L-1 for 24 h. CONCLUSION: Bilobalide induced GDNF and VEGF expression in the cultured astrocytes. |
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