茜草双酯对小鼠免疫功能的抑制作用(英文)

目的:研究茜草双酯对正常小鼠免疫功能的影响.方法:采用免疫药理学常用方法即血清溶菌酶含量的测定,迟发型超敏反应的测定,血清溶血素的测定,[~3H]TdR参入的小鼠全血白细胞吞噬能力的测定,鼠脾空斑形成细胞溶血能力的测定,T和B淋巴细胞转化能力的测定.结果:茜草双酯125,500,2000mg·kg~(-1)·d~(-1)降低血清溶菌酶含量和全血白细胞吞噬功能;使PFC溶血能力和HC_(50)产生减少;抑制DTH反应;体内给药体外测定,抑制[~3H]TdR参入的PHA与LPS诱导的淋巴细胞转化.以上作用呈一定的剂量依赖性.结论:茜草双酯对正常小鼠特异和非特异性免疫功能均有不同程度的抑制作用.

Immunosuppressive effects of rubidatum in mice

AIM: To study the effect of rubidatum (Rub) on immune function in normal mice. METHODS: Serum lysozyme concentration (SLC) was measured using micrococcus lysodiekticus as a substrate. Delayed type hypersensitivity (DTH) was determined by measuring the thickness of the right hind footpad 24 h after the injection of 1 x 10(8) washed sRBC (50 microL 10% sRBC). Serum hemolysin concentration was determined by OD measuring at A540 after the serum was treated with 2-mercaptoethanol. Phagocytic function of peripheral leukocyte (Leu) were determined by the incorporated radioactivity of [3H]TdR. The hemolytic activity of plaque forming cell (PFC) was determined by measuring the lymphocytemediated hemolysis of sheep red blood cell in vitro. T- and B-lymphocyte transformation (TLT and BLT) were induced by phytohemagglutinin (PHA) and lipopolysacharide (LPS) respectively and measured by the incorporation of [3H]TdR. RESULTS: Rub 125, 500, 2000 mg.kg-1.d-1 p.o. to BALB/c (or NIH) mice decreased the SLC; inhibited the phagocytosing functions of peripheral leukocytes; diminished the hemolytic activity of PFC; decreased the HC50; inhibited the DTH reaction; and showed inhibitory effects on TLT and BLT. CONCLUSION: Rub has immunosuppressive effects on immune system in mice by affecting M phi, T, and B lymphocyte, which suggests that Rub has inhibitory effects on both nonspecific and specific immune function.