| 10—羟基喜树碱对人肝癌Hep G2细胞的分化诱导作用 |
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| 引用本文： | Zhang XW,Jiang JF,Xu B. 10—羟基喜树碱对人肝癌Hep G2细胞的分化诱导作用[J]. Acta pharmacologica Sinica, 2000, 21(4): 364-368 |
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| 作者姓名： | Zhang XW Jiang JF Xu B |
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| 作者单位： | 中国科学院上海药物研究所药理室,中国科学院上海药物研究所药理室,中国科学院上海药物研究所药理室 上海 200031 中国,上海 200031 中国,上海 200031 中国 |
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| 基金项目： | Project supported by the National Natural Science Foundation of China, № 39570824 |
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| 摘 要： | 目的:研究羟基喜树碱HCPT对人肝癌Hep G2细胞分化诱导作用的机制。方法:用免疫细胞化学染色检测增殖细胞核抗原表达;流式细胞仪检测细胞周期和野生型p53蛋白表达;端粒重复扩增法检测端粒酶活性。结果:以诱导分化剂量(5-20μg·L~(-1))的HCPT处理6 d,人肝癌Hep G2细胞明显受阻于G_2/M期,PCNA阳性表达率降低。经HCPT 5μg·L~(-1)处理6 d,Hep G2细胞的野生型p53蛋白表达明显增强,但端粒酶活性没有改变。结论:HCPT诱导人肝癌Hep G2细胞分化的作用与细胞阻滞于G_2/M期、PCNA表达降低及野生型p53蛋白表达增强有关。
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| 关 键 词： | 10－羟基喜树碱 肝肿瘤 细胞分化 细胞周期 p53蛋白 免疫组织化学 增殖细胞核抗原 端粒酶 流式细胞术 |
Differentiation-inducing action of 10-hydroxycamptothecin on human hepatoma Hep G2 cells |
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| Zhang X W,Jiang J F,Xu B. Differentiation-inducing action of 10-hydroxycamptothecin on human hepatoma Hep G2 cells[J]. Acta pharmacologica Sinica, 2000, 21(4): 364-368 |
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| Authors: | Zhang X W Jiang J F Xu B |
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| Affiliation: | Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | AIM: To study the mechanism of differentiation-inducing action of 10-hydroxycamptothecin (HCPT) on human hepatoma Hep G2 cells. METHODS: The proliferating cell nuclear antigen (PCNA) expression was studied by immunocytochemical staining method. The cell cycle distribution and the wild-type protein p53 expression were measured by flow cytometry. Telomerase activity was assayed with telomeric repeat amplification protocol (TRAP). RESULTS: After treatment with HCPT at differentiation-inducing concentrations 5-20 micrograms.L-1 for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 micrograms.L-1 for 6 d, the p53 expression level markedly increased in comparison with the control cells. The telomerase activity did not change in Hep G2 cells treated with HCPT 5-20 micrograms.L-1 for 8 d. CONCLUSION: The differentiation-inducing effect of HCPT on Hep G2 cells is related with the cell cycle arrest at G2/M phase, down-regulation of PCNA and up-regulation of wild-type protein p53. |
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| Keywords: | 10-hydroxycamptothecin experimental liver neoplasm cell differentiation cell cycle protein p53 immunohistochemistry proliferating cell nuclear antigens telomerase flow cytometry |
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