左旋千金藤立定诱导6-羟基多巴胺损毁大鼠的纹状体Fos、前脑啡肽mRNA、前强啡肽mRNA表达与旋转行为的关系(英文)

目的:研究左旋千金藤立定(SPD)调节6-羟基多巴胺(6-OHDA)损毁大鼠纹状体的Fos、前脑腓肽(PENK)和前强啡肽(PDYN)mRNA表达水平与旋转行为的关系.方法:用免疫细胞化学法观察Fos表达,用地高辛非同位素法标记的寡核苷酸探针检测纹状体PENK和PDYN mRNA,进行图像处理作半定量分析.结果:(1)SPD给予1,3,7 d后,损毁大鼠旋转行为仍维持在高水平;(2)SPD诱导双侧纹状体Fos显著表达,尤以损侧为甚.SPD重复应用使双侧纹状体的Fos诱导表达下降,尤以健侧为显著;(3)与健侧相比,损毁侧纹状体的PENK mRNA表达水平增加非常显著.SPD重复应用7 d,使这种增加的PENK mRNA水平明显下降.同时,SPD也使健侧PENK mRNA水平降低.然而,6-OHDA损毁和SPD处理对双侧纹状体的PDYN mRNA水平无明显影响.结论:SPD激发6-OHDA损毁大鼠旋转行为维持在高水平,与损侧纹状体的Fos表达和PENK mRNA水平下降是同步的.但是,6-OHDA损毁和SPD均未显示出对PDYN mRNA表达的影响.

Relevance between striatal expression of Fos, proenkephalin mRNA, prodynorphin mRNA and rotation induced by l-stepholidine in 6-hydroxydopamine-lesioned rats

AIM:To study that l-stepholidine(SPD) regulates the expression of proenkephalin (PENK) and prodynorphin (PDYN) mRNA and Fos in the striatum after rotational test in the 6-hydroxydopamine (6-OHDA)-lesioned rats. METHODS:PENK and PDYN mRNA levels were examined with in situ hybridization, and Fos expression was detected with immunocytochemistry. The data were semi-quantified with image analyzer. RESULTS:(1) Following repeated SPD treatment, the rotation was kept on high activity in the 6-OHDA-lesioned rats.(2) SPD significantly elicited Fos expression in both sides of striatum, particularly in the denervated one.Repeated administration of SPD, Fos expression declined on both sides, particularly in the intact one. (3)In the denervated striatum of 6-OHDA-lesioned rats, the PENK mRNA level was extremely increased vs that in the intact striatum. This high level of PENK mRNA was significantly reduced by 7-d treatments of SPD. SPD also reduced the level of PENK mRNA in the intact striatum. However, the level of PDYN mRNA did not show significant change in both sides of striatum after denervation or SPD treatment. CONCLUSION: In the 6-OHDA-lesioned rats, the rotation induced by SPD was kept on a high activity, which was in pace with the inducement ofFos expression and the reduction of expression of PENK mRNA in the denervated striatum. But then the lesion and SPD treatment had no remarkable effect on the expression of PDYN mRNA.