酸性菟丝子多糖的化学特征和免疫活性(英文)

目的:研究从菟丝子(Cuscuta chinensis Lam)中提取分离的酸性纯多糖CHC-1的结构特征及免疫活性.方法:利用化学方法和NMR光谱分析方法对CHC-1的结构特征进行了解析,并研究了CHC-1对小鼠抗体生成以及对淋巴细胞增殖率的影响.结果:CHC-1的分子量大于1.0×10~6.糖组份分析结果显示CHC-1是由Rha,Ara,Gal和GalA组成,比例为:0.8:1.0:1.5:0.3.甲基化结果和~1H,~(13)C NMR分析进一步确定了CHC-1中各残基的连接方式.CHC-10.1 g/L能在体外明显升高T,B细胞的增殖率,CHC-125,50 mg/kg显著增加小鼠的脾重,淋巴细胞的增殖率以及促进抗体生成.结论:CHC-1为一种多分支的杂多糖,并具有免疫增强活性.

Chemical characterization and immunological activities of an acidic polysaccharide isolated from the seeds of Cuscuta chinensis Lam

AIM: To study chemical characterization and immunological activities of an acidic polysaccharide, CHC-1, isolated and purified from the seeds of Cuscuta chinensis Lam. METHODS: Both chemical and spectral methods were used to investigate the chemical characterization of CHC-1. Effect of CHC-1 on the proliferation rates of T- and B-lymphocytes both in vivo and in vitro, and antibody production in vivo was measured at various concentrations of CHC-1. RESULTS: The molecular weight of CHC-1 was estimated to be more than 1.0 x 10(6). The analytical results of sugar components indicated that CHC-1 was composed of Rha, Ara, Gal, and GalA in a molar ratio of 0.8:1.0:1.5:0.3. Methylation analysis and 1H, 13C NMR further identified the linkages of the residues of CHC-1. CHC-1 0.1 g/L promoted remarkably the proliferation of T-cells and B-cells in vitro. CHC-1 25 mg/kg, 50 mg/kg caused an evident increase in spleen weight, lymphocyte proliferation, antibody production, etc. But its effect on IgG levels was not significant. CONCLUSION: CHC-1 is a highly branched heteropolysaccharide and possessed immune enhancement activities.