| 二甲基氨氯吡咪诱导HL-60细胞分化(英文) |
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| 引用本文： | Li YY,Liang NC,Jiang L,Wu T,Lin XY. 二甲基氨氯吡咪诱导HL-60细胞分化(英文)[J]. Acta pharmacologica Sinica, 2000, 21(5): 445-449 |
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| 作者姓名： | Li YY Liang NC Jiang L Wu T Lin XY |
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| 作者单位： | 广东医学院医用生化研究所,广东医学院医用生化研究所,广东医学院医用生化研究所,广东天然药物研究开发重点实验室,广东天然药物研究开发重点实验室 湛江 524023 中国,湛江 524023 中国,湛江 524023 中国,湛江 524023 中国,湛江 524023 中国 |
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| 基金项目： | Project support by Guangdong Research Foundation for Key Field.№ 9306. |
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| 摘 要： | 目的:研究阿米洛利衍生物二甲基阿米洛利(DMA)对培养的HL-60细胞增殖和分化的影响。方法:MTT法测定细胞增殖,细胞染色和NBT还原试验测定细胞分化。结果:DMA以浓度依赖方式抑制HL-60细胞增殖,药物作用96小时测得半数抑制浓度(IC_(50))为31.7(95％可信限为6.3-57.1)μmol·L~(-1)。DMA诱导HL-60细胞向粒细胞系分化。加入DMA100μmol·L~(-1)作用3天,细胞分化率从6.5％升至70％。阿米洛利及阿米洛利的另二个衍生物对细胞分化无明显作用。结论:二甲基阿米洛利抑制HL-60细胞增殖和诱导HL-60细胞分化。
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| 关 键 词： | 细胞分裂 细胞分化 HL-60细胞 阿米洛利 半数抑制浓度 |
Dimethylamiloride-induced differentiation of HL-60 cells |
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| Li Y Y,Liang N C,Jiang L,Wu T,Lin X Y. Dimethylamiloride-induced differentiation of HL-60 cells[J]. Acta pharmacologica Sinica, 2000, 21(5): 445-449 |
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| Authors: | Li Y Y Liang N C Jiang L Wu T Lin X Y |
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| Affiliation: | Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, Guangdong 524023, China. Guangdong Key Lab for Research & Development of Natural Medicines, Zhanjiang, Guangdong 524023, China. liyunyuan@163.net |
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| Abstract: | AIM: To study the effect of 5-(N,N-dimethyl) amiloride (DMA) on the proliferation and differentiation of HL-60 cells in vitro. METHODS: MTT assay to test cytotoxicity; cell staining and NBT reduction to test cell differentiation. RESULTS: DMA inhibited HL-60 cells growth in a concentration-dependent manner, and IC50 value for 96 h was 31.7 (95% confidence limits: 6.3-57.1) mumol.L-1. DMA also induced granulocytic differentiation in HL-60 cells. The percentage of differentiating cells increased from 6.5% to 70% after DMA 100 mumol.L-1 treatment for 3 d. The differentiating effect of DMA was distinguishable from amiloride, 5-(N-ethyl-N-isopropyl)amiloride (EIPA), and (5-(N-ethyl-N-isopropyl)amiloride (MIA). None among the amiloride, EIPA, and MIA were capable of triggering the differentiation of HL-60 cells. CONCLUSION: DMA inhibited the proliferation of HL-60 cells and induced differentiation of HL-60 cells. |
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| Keywords: | cell division cell differentiation HL-60 cells amiloride inhibitory concentration 50 |
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