人白介素16与HIV-1的CD4受体的相互作用(英文)

目的:研究人白介素-16和HIV-1病毒受体CD4(T淋巴细胞分化抗原)的作用机理。方法:人白介素-16的结构保守区由SYBYL软件中的Biopolymer模块建立,其非保守区由LOOP SEARCH方法建立。结果:人白介素-16首先形成二聚体,然后与CD4的二聚体进一步形成HIL-16与CD4的复合物二聚体。接着,该复合物二聚体通过二硫键(Cys31-Cys31)形成HIL-16与CD4复合物的四聚体。结论:该相互作用模型有助于推测白介素-16的作用机制,也有益于全新抗艾滋病药物的合理设计。

Interaction between human interleukin-16 and CD4 receptor of HIV-1

AIM: To study the interaction between human inter-leukin-16 (IL-16) and the receptor CD4 (T-lymphocyte differentiation antigen) of human immunodeficiency virus type 1 (HIV-1). METHODS: Two structurally conserved regions (SCRs) of human IL-16 were built by the SYBYL/Biopolymer module using the corresponding transmembrane (TM) domain of human interleukin-4 (HIL-4) and HIL-2 as the templates. The coordinates for amino-terminal residue sequence, carboxyl-terminal residue sequences, and cytoplasm loops were generated using Biopolymer's LOOP SEARCH algorithm. RESULTS: HIL-16 first formed a homodimer, then contacted with CD4 dimer further forming a dimeric complex. Subsequently, the dimeric complex constructed the tetrameric complex by two disulfide bridges between the cysteines of MIL-16 (Cys31 -Cys31). CONCLUSION: The interaction model is useful to propose the action mechanism of HIL-16 and is beneficial for rational designing of novel anti-HTV drugs.