1-甲基-3-异丁基黄嘌呤延迟去除生长因子诱导的血管内皮细胞凋亡(英文)

目的:研究1-甲基-3-异丁基黄嘌呤(MIBX)对去除生长因子(aFGF和血清)诱导的血管内皮细胞凋亡的影响.方法:通过细胞存活率的分析,荧光显微技术和DNA凝胶电泳等方法,检测MIBX对细胞凋亡的影响.结果:用25-200μmol/L的MIBX处理培养在无aFGF和血清的培养液中的血管内皮细胞,50-200μmol/L的MIBX在处理6h明显抑制了凋亡小体的形成和DNA的片断化.但是同样浓度的MIBX处理细胞12h以后,处理组和对照组之间无明显差别.结论:MIBX延迟去除aFGF和血清诱导的血管内皮细胞凋亡.

1-Methyl-3-isobutylxanthine delays apoptosis induced by deprivation of growth factors in vascular endothelial cells

AIM: To investigate the effects of 1-methyl-3-isobutylxanthine (MIBX) on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells (VEC). METHODS: Nuclear fragmentation was observed by fluorescence microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was measured by agarose gel electrophoresis. RESULTS: The cells deprived of aFGF and serum were treated with MIBX 25-200 mumol/L for 3, 6, 9, and 12 h, respectively. Morphological changes including the formation of apoptotic bodies and DNA fragmentation of these cells were significantly suppressed by IBMX 50-200 mumol/L at 3 h and 6 h. But after 12-h treatment, no difference was observed between the treated and untreated cells. CONCLUSION: MIBX delays apoptosis of vascular endothelial cells induced by deprivation of aFGF and serum.