套式聚合酶链反应检测巨细胞病毒的初步研究

目的建立套式聚合酶链反应（ＰＣＲ）加限制性酶切分析方法检测人巨细胞病毒（ＨＣＭＶ）。方法在ＨＣＭＶ直接早期蛋白ＥｃｏＲＩＪＤＮＡ片段内，自行设计两对引物，建立套式ＰＣＲ检测ＨＣＭＶＤＮＡ，同时结合病毒分离检测临床标本。结果２３例新生儿肝炎综合征患儿中，１０例病毒分离及套式ＰＣＲ均阳性；１例病毒分离阴性，但套式ＰＣＲ阳性。对５８例妊娠早期孕妇血标本进行检测，套式ＰＣＲ阳性率为９％，病毒分离阳性率则为７％。结论套式ＰＣＲ加限制性酶切分析是一种临床检测ＨＣＭＶ的快速有效手段，值得临床推广。

The detection of human cytomegalovirus with nested polymerase chain reaction

Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics. Method We designed two primer sequences from the DNA fragment coding the immediate early protein Eco RI J and established nested PCR for detecting HCMV DNA. Meanwhile, nested PCR and virus isolation were used to detect HCMV in clinic samples.Results Among 23 samples of neonatal jaundice syndrome, HCMV was found in 10 by using nested PCR and virus isolation, one was negative with virus isolation, but positive with nested PCR. Among 58 samples of early gravida, the positive rate of nested PCR, virus isolation were 8.6% and 6.9%, repectively. Conclusion Nested PCR addition with restrictive enzyme may be used for rapid detection of clinical infection of HCMV.