严重烫伤后脑内GFAP表达变化与血脑屏障功能的关系

目的:探讨严重烫伤后星形胶质细胞的形态学变化、GFAP表达与血脑屏障功能的关系。方法:建立严重烫伤大鼠模型。健康SD大鼠,随机分为正常组,假烫伤组,烫伤组,烫伤后又设3、6、12、24、48h5个时间点,用透射电镜观察血脑屏障超微结构变化;紫外分光光度计检测脑伊文蓝含量。免疫组化观察脑GFAP表达。结果:烫伤后星形胶质细胞线粒体肿胀、空泡化,胶质细胞有凋亡期表现。脑EB含量明显增高,GFAP表达增加(P<0.01)。结论:严重烫伤后星形胶质细胞活化、GFAP表达增加与血脑屏障通透性增高有关,提示GFAP在严重烫伤早期血脑屏障的损伤中起重要作用。

Changes of GFAP expression and blood-brain barrier permeability after severe scald in rats

Objective： To explore the morphologic change of astrocyte , GFAP expression and its relationship with blood-brain barrier permeability after severe scald in rats. Methods： The model of burn was established. Healthy SD rats were divided into three groups ： normal control ,sham-scalded group ,scald group, scald group were quantified at 3, 6, 12, 24,and 48 h post thermal injury. The ultrastructural change of blood brain barrier was observed by transmission electron microscope; Brain Evans blue content was examined with the ultraviolet spectrophotometer; The change of brain GFAP expression after severe scald was determined by immunohistochemieal method. Results： Under transmission electron microscope, mitochondrions of astrocytes of the cerebral cortex were swollen and vacuolated, Brain Evans blue content and expression of GFAP were significantly increased after burn （P 〈 0. 01）. Conclusion： The increase in GFAP expression, astroeyte activation, associated with increased EBB permeability following thermal injury, indicates that GFAP may contribute to EBB disruption in peripheral thermal injury.