广西4地带绦虫分离株COX1序列分析

目的 分析广西4地带绦虫分离株COX1序列， 了解亚洲绦虫在广西的分布。方法 对鹿寨、 融水、 田东、 三江等 4县现症病人进行驱虫， 收集绦虫成虫。绦虫成虫经反复洗涤后， 各取孕节一节， 研碎后提取基因组DNA； 同时设计特异性引物， PCR扩增各分离株COX1片段， 对扩增产物进行T?A克隆测序； 应用相关软件分析序列的同源性、 遗传距离， 同时构建系统发生树。同时， 从GenBank检索亚洲绦虫、 猪带绦虫和牛带绦虫COX1基因序列， 并与4地分离株基因序列进行比较。结果 广西4地5个带绦虫分离株的COX1碱基序列长度均为444 bp。鹿寨株与亚洲绦虫之间基因差异为5个位点（1.11%）， 同源性为98.87%， 遗传距离为0.011， 其在邻接法和简约法构建的系统发生树中位于同一分枝。融水A株与猪带绦虫同源性达100%， B株与牛带绦虫和亚洲绦虫的同源性分别达98.20%和96.17%； 田东和三江分离株与猪带绦虫的同源性分别为99.55%和96.40%， 遗传距离分别为0.005和0.037； 鹿寨株与牛带绦虫的同源性达96.40%。结论 广西鹿寨县存在亚洲绦虫流行， 融水县存在牛带绦虫和猪带绦虫混合流行。

Analysis of COX1 sequences of Taenia isolates from four areas of Guangxi

Objective To analyze the COX1 sequences of Taenia isolates from four areas of Guangxi Zhuang Autonomous Region,and to understand the distribution of Taenia asiatica in Guangxi.Methods Patients with taeniasis in Luzhai,Rongshui,Tiandong and Sanjiang in Guangxi were treated by deworming,and the Taenia isolates were collected.Cyclooxygenase-1(COX1)sequences of these isolates were amplified by PCR,and the PCR products were sequenced by T-A clone sequencing.The homogeneities and genetic distances were calculated and analyzed,and the phylogenic trees were constructed by some softwares.Meanwhile,the COX1 sequences of the isolates from the 4 areas were compared separately with the sequences of Taenia species in GenBank.Results The COX1 sequence of the 5 Taenia isolates collected had the same length of 444 bp.There were 5 variable positions between the Luzhai isolate and Taenia asiatica,the homogeneity was 98.87% and their genetic distance was 0.011.The phylogenetic tree analysis revealed that the Luzhai isolate and Taenia asiatica locating at the same node had a close relationship.The homogeneity between Rongshui isolate A and Taenia solium was 100%,while the homogeneity of Rongshui isolate B with Taeniasis saginata and Taenia asiatica were 98.20% and 96.17%,respectively.The homogeneities of the Tiandong and Sanjiang isolates with Taenia solium were 99.55% and 96.40%,respectively,and the genetic distances were 0.005 and 0.037,respectively.The homogeneity between the Luzhai isolate and Taeniasis saginate was 96.40%.Conclusion Taenia asiatica exists in Luzhai and Taenia solium and Taenia saginata coexist in Rongshui,Guangxi Zhuang Autonomous Region.