AQP1和AQP3在子宫颈鳞状细胞癌中表达的研究

目的:探讨AQP1和AQP3在子宫颈癌中的表达及意义。方法:通过荧光定量PCR和免疫荧光检测AQP1和AQP3在人子宫颈癌SiHa细胞系中的表达,通过免疫组化检测AQP1和AQP3在35例维吾尔族子宫颈癌、15例CINⅢ和15例慢性宫颈炎中的表达。结果:(1)SiHa细胞中AQP1和AQP3在mRNA和蛋白水平均表达;(2)AQP1表达于宫颈病变组织间质血管内皮细胞的胞质,采用微血管密度(MVD)表示AQP1表达强度。慢性宫颈炎、CINⅢ和子宫颈癌组的MVD分别是43.6±17.8、56.2±11.6、70.8±21.1,宫颈癌组MVD显著高于CINⅢ组及慢性宫颈炎组(P均<0.05),CINⅢ和慢性宫颈炎组间差异无统计学意义;AQP3在慢性宫颈炎、CINⅢ和子宫颈癌组的阳性率分别是13.33%、26.67%、48.57%,子宫颈癌组与慢性宫颈炎组间差异有统计学意义(P=0.018),子宫颈癌组与CINⅢ、CINⅢ与慢性宫颈炎组间差异均无统计学意义(P均>0.05)。结论:AQP1和AQP3的表达可能与子宫颈癌的发生、发展有关。

Expression of AQPI and AQP3 in cervical squamous cell carcinoma

Objective：To explore the expression and significance of AQP1 and AQP3 in cervical carcinoma. Methods： Expression of AQP1 and AQP3 in SiHa cell line was examined by RT-PCR and immunofluorescence,and in 35 cases of cervical carcinoma,15 cases CINⅢ and 15 cases of chronic cervicitis by immunohistochemistry. Results： （ 1） AQP1 and AQP3 expressed in SiHa cell line; （2） AQP1 expressed in the cytoplasm of stromal vascular endothelial cells in cervical lesions,MVD was used as representing AQP1 expression; MVD were 43. 6±17. 8,56. 2±11. 6,70. 8±21. 1 in chronic cervicitis group,CINⅢ group and cervical carcinoma group,respectively . MVD in cervical carcinoma was significantly higher than that in CIN Ⅲand chronic cervicitis（P0.05） ,but difference of MVD between CIN Ⅲ and chronic cervicitis was not significant（ P0. 05） . Positive rates of AQP3 were 13. 33% ,26. 67% ,48. 57% ,respectively,in chronic cervicitis group,CINⅢ group and cervical carcinoma group. Difference of AQP3 expression between cervical carcinoma group and chronic cervicitis group was significant（P0.05） ,and difference between cervical carcinoma group and CINⅢ ,CINⅢ and chronic cervicitis group was not significant（P0.05） . Conclusion： Expression of AQP1 and AQP3 in cervical carcinoma may be related with cervical carcinogenesis and development.